Environmental Health and Preventive Medicine

, Volume 3, Issue 4, pp 215–217 | Cite as

Serum leptin levels and their association with several factors related to arteriosclerosis among medical students in Japan

  • Motonobu Miyazaki
  • Akihiko Kaetsu
  • Yoshito Momose
  • Hiroshi Une
Original Article


The association between serum leptin levels and several factors related to arteriosclerosis were studied in subjects who were Japanese medical students taking no medications. The group was comprised of 75 males and 35 females. The age distribution in males was 21.4 to 29.8 years (median age, 24.0 years) and in females was 21.3 to 29.9 years (median age, 22.9 years). Statistical analyses were performed using the Wilcoxon rank sum test and Spearman correlation. Median levels of serum leptin were 3.3 ng/ml in males and 7.6 ng/ml in females. The largest correlation observed was widi percent body fat irrespective of sex [males; r=0.775, p<0.001, females; r=0.553, p<0.001]. However, body mass index (BMI) was similarly well correlated with serum leptin [r=0.631, p<0.001] in males but not in females [r=0.305, p=0.075]. A negative correlation was observed between high density lipoprotein cholesterol (HDL-C) and leptin in both sexes [males; r=-0.298, p<0.01, females; r=-0.363, p<0.05] .respectively. Percent body fat, BMI, diastolic blood pressure, apolipoprotein B, and HDL-C were the most significant factors in males. Percentage of body fat and HDL-C were the most important factors in females.

Key words

serum leptin medical students sex difference percent body fat HDL-C 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1).
    Zhang Y, Proenca R, Maffei M, Barone M, Leopord L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature 1994; 372:425–32.PubMedCrossRefGoogle Scholar
  2. 2).
    Pelleymounter MA, Cullen MJ, Baker MB, et al. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 1995; 269: 540–3.PubMedCrossRefGoogle Scholar
  3. 3).
    Caprio S, Tamborlane WV, Silver D, et al. Hyperleptinemia: an early sign of juvenile obesity. Relations to body fat depots and insulin concentrations. Am J Physiol 1996; 34: E626–30.Google Scholar
  4. 4).
    Maffei M, Halaas J, Ravussin E, et al. Leptin levels in human and rodent: Measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med 1995; 1: 953–6.CrossRefGoogle Scholar
  5. 5).
    Considine RV, Sinha MK, Heiman ML, et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. N Engl J Med 1996; 334: 292–5.PubMedCrossRefGoogle Scholar
  6. 6).
    Ostlund RE, Yang JW, Klein S, Gingerich R. Relationship between plasma leptin concentrations and body fat, gender, diet, age, and metabolic covariates. J Clin Endocrinol Metab 1996; 81: 3909–13.PubMedCrossRefGoogle Scholar
  7. 7).
    Zimmet P, Hodge A, Nicolson M, Gingerich RE. Serum leptin concentration, obesity, and insulin resistance in Western Samoans: cross sectional study. Brit Med J 1996; 313: 965–9.PubMedGoogle Scholar
  8. 8).
    Sato T. Bio-electrical impedance analysis method. Jpn J Clin Med 1995; 53: 179–82 (in Japanese).Google Scholar
  9. 9).
    Sakamoto Y, Sato T, Ohno M, Ikeda Y. Evaluation on the body fat by bioelectrical impedance analysis. JASSO 1992; 13: 279–80 (in Japanese).Google Scholar
  10. 10).
    Frohlich ED, Grim C, Labarthe DR, Maxwell MH, Perloff D, Weidman WH. Recommendations for human blood pressure determination by sphygmomanometers. Circulation 1988; 77: 502A-14AGoogle Scholar
  11. 11).
    Ma Z, Gingerich R, Santiago JV, Klein S, Smith Ch, Laudt M. Radioimmunoassay of leptin in human plasma. Clin Chem 1996; 42: 942–6.PubMedGoogle Scholar
  12. 12).
    Noma A, Hat Y, Goto Y. Quantitation of serum apolipoprotein A-I, A-II, B, C-II, C-III and E in healthy Japanese by turbidimetric immunoassay: reference values, and age- and sex-related differences. Clinica Chimica Acta 1991; 199: 147–58.CrossRefGoogle Scholar
  13. 13).
    Ikeda Y. Definition and diagnosis on obesity. JASSO 1992; 13: 64–6 (in Japanese).Google Scholar
  14. 14).
    Haffner SM, Gingerich RL, Miettinen H, Stern MP. Leptin concentrations in relation to overall adiposity and regional body fat distribution in Mexican Americans. Int J Obese 1996; 20: 904–8.Google Scholar

Copyright information

© Japanese Society of Hygiene 1999

Authors and Affiliations

  • Motonobu Miyazaki
    • 1
  • Akihiko Kaetsu
    • 2
  • Yoshito Momose
    • 1
  • Hiroshi Une
    • 1
  1. 1.Department of Hygiene and Preventive Medicine, School of MedicineFukuoka UniversityFukuoka City, FukuokaJapan
  2. 2.Department of Public Health, School of MedicineFukuoka UniversityFukuoka

Personalised recommendations