Abstract
Immunoproteomic analysis was applied to study the immunoreactivity of serum samples collected at different time points from a laboratory assistant accidentally infected with highly virulent strain ofFrancisella tularensis subsp.tularensis. Immunoblotting showed that the spectrum ofF. tularensis antigens recognized specifically by immune sera remained with the exception for 1 antigen stable for up to 16 years after infection. Using immunoproteomics approach 10 immunoreactive antigens were successfully identified. Several new immunogenicF. tularensis proteins were described for the first time.
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Abbreviations
- F.t. :
-
Francisella tularensis
- F.t.t. :
-
Francisella tularensis subsp.tularensis
- F.t.h. :
-
Francisella tularensis subsp.holarctica
- 2-DE:
-
2-dimensional electrophoresis
- LVS:
-
live vaccine strain
- MS:
-
mass spectrometry
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This study was supported by the grant MO0FVZ 0000501 from theMinistry of Defense of the Czech Republic.
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Janovská, S., Pávková, I., Reichelová, M. et al. Proteomic analysis of antibody response in a case of laboratory-acquired infection withFrancisella tularensis subsp.tularensis . Folia Microbiol 52, 194–198 (2007). https://doi.org/10.1007/BF02932159
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DOI: https://doi.org/10.1007/BF02932159