Folia Microbiologica

, Volume 52, Issue 2, pp 194–198 | Cite as

Proteomic analysis of antibody response in a case of laboratory-acquired infection withFrancisella tularensis subsp.tularensis

  • S. Janovská
  • I. Pávková
  • M. Reichelová
  • M. Hubálek
  • J. Stulík
  • A. Macela


Immunoproteomic analysis was applied to study the immunoreactivity of serum samples collected at different time points from a laboratory assistant accidentally infected with highly virulent strain ofFrancisella tularensis subsp.tularensis. Immunoblotting showed that the spectrum ofF. tularensis antigens recognized specifically by immune sera remained with the exception for 1 antigen stable for up to 16 years after infection. Using immunoproteomics approach 10 immunoreactive antigens were successfully identified. Several new immunogenicF. tularensis proteins were described for the first time.


Immune Seron Tularemia Laboratory Assistant Francisella Tularensis Intracellular Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Francisella tularensis


Francisella tularensis subsp.tularensis


Francisella tularensis subsp.holarctica


2-dimensional electrophoresis


live vaccine strain


mass spectrometry


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • S. Janovská
    • 1
  • I. Pávková
    • 1
  • M. Reichelová
    • 2
  • M. Hubálek
    • 1
  • J. Stulík
    • 1
  • A. Macela
    • 1
  1. 1.Institute of Molecular Pathology, Faculty of Military Health SciencesUniversity of DefenseHradec KrálovéCzechia
  2. 2.Veterinary Research InstituteBrnoCzechia

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