Folia Microbiologica

, 51:183 | Cite as

Demonstration of the shorter flagellin (flaA) gene of urease-positive thermophilicCampylobacter isolated from the natural environment in Northern Ireland

  • T. Gondo
  • T. Sekizuka
  • N. Manaka
  • O. Murayama
  • B. C. Millar
  • J. E. Moore
  • M. Matsuda


The PCR amplicons (about 1450 bp in length) offlaA gene fragments of 11 isolates of urease-positive thermophilicCampylobacter (UPTC) isolated from the natural environment not including wild birds in Northern Ireland were demonstrated to be shorter than those ofC. jejuni 81116 and six isolates ofC. jejuni andC. coli (about 1700 bp) isolated in Northern Ireland and Japan. When the nucleotide lengths of the possible open reading frame (ORF) of theflaA genes were determined, those from the 11 UPTC isolates were estimated to be 1464–1503 bp, and those from the sixC. jejuni andC. coli isolates andC. jejuni 81116 strain to be 1716–1728 bp. Nucleotide sequence and deduced amino acid sequence alignments of the possible ORFs demonstrated that the ORFs from the 11 UPTC isolates lack about 80 amino acid residues, mainly from the approximate residue numbers 390–470 of the large variable region in theflaA protein of the seven isolates ofC. jejuni andC. coli, and do not have any internal termination codons. High amino acid sequence similarity of both amino- and carboxy-termini of the ORFs of theflaA gene was demonstrated between the 11 isolates of UPTC and the 7 isolates ofC. jejuni andC. coli. The 11 UPTC isolates examined were strongly suggested to possess a shorterflaA gene without any internal termination codons.


Wild Bird Flagellin Gene Southern Blot Hybridization Analysis Large Variable Region High Amino Acid Sequence Similarity 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2006

Authors and Affiliations

  • T. Gondo
    • 1
  • T. Sekizuka
    • 1
  • N. Manaka
    • 1
  • O. Murayama
    • 1
  • B. C. Millar
    • 2
  • J. E. Moore
    • 2
  • M. Matsuda
    • 1
  1. 1.Laboratory of Molecular Biology, School of Environmental Health SciencesAzabu UniversitySagamiharaJapan
  2. 2.Department of Bacteriology, Northern Ireland Public Health LaboratoryBelfast City HospitalBelfastUK

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