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Folia Microbiologica

, 52:525 | Cite as

Neisseria meningitidis strains from patients with invasive meningococcal disease differ in stimulation of cytokine production

  • M. Holub
  • M. Scheinostová
  • O. Džupová
  • A. Fišerová
  • O. Beran
  • J. Kalmusová
  • M. Musílek
  • P. Křížová
Article

Abstract

Cytokine production was determinedin vitro after stimulation with three differentNeisseria meningitidis (NM) strains. Virulent NM B strain isolated from a patient with mild course of invasive meningococcal disease (IMD) elicited higher cytokine production than NM B and NM C hypervirulent strains isolated from patients with moderate and fatal course of IMD, respectively. Endotoxin concentration afterin vitro stimulation correlated with cytokine production: the highest endotoxin levels were observed with virulent NM B strain. Serum cytokines and endotoxin levels showed an opposite trend. These results suggest that inflammatory response during IMD is predominantly influenced by host factors.

Keywords

Cytokine Production Meningococcal Disease Neisseria Meningitidis Endotoxin Level Endotoxin Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

APACHE

acute physiological and chronic health evaluation

CRP

C-reactive protein

FC

flow cytometer

FMS

fulminant meningococcal sepsis

IL

interleukin

IMD

invasive meningococcal disease

LOS

lipooligosaccharide

MLST

multilocus sequence typing

NM

Neisseria meningitidis

rSBA

serum bactericidal assay with the use of baby rabbit complement

SOFA

sepsis organ failure assessment

TNF-α

tumor necrosis factor α

WB

whole blood

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • M. Holub
    • 1
  • M. Scheinostová
    • 1
  • O. Džupová
    • 2
  • A. Fišerová
    • 1
  • O. Beran
    • 1
  • J. Kalmusová
    • 3
  • M. Musílek
    • 3
  • P. Křížová
    • 3
  1. 1.3rd Department of Infectious and Tropical Diseases, 1st Faculty of MedicineCharles University in PraguePragueCzechia
  2. 2.Department of Infectious Diseases, 3rd Faculty of MedicineCharles University in PraguePragueCzechia
  3. 3.National Reference Laboratory for Meningococcal InfectionsNational Institute of Public HealthPragueCzechia

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