Skip to main content

Existence ofSinorhizobium meliloti genomic fragment hybridizing with thenifM gene ofKlebsiella pneumoniae

Folia Microbiologica volume 52, pages 447–448 (2007)Cite this article

Abstract

A novel finding that genomic restriction fragments of symbiotic nitrogen fixerS. meliloti hybridized withnifM gene probe of the free-living diazotrophKlebsiella pneumoniae is reported. WhenSmaI endonuclease was used to digestS. meliloti DNA, a unique hybridizing band was obtained.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price includes VAT (USA)
Tax calculation will be finalised during checkout.

References

  • Burgess B.K.: pp. 103–104 in C. Veeger, W.E. Newton (Eds):Advances in Nitrogen Fixation Research. Nijhoff/Junk, The Hague 1984.

    Google Scholar 

  • Evans D., Jones R., Woodley P., Wilborn R., Robson R.: Nucleotide sequence and genetic analysis of theAzotobacter chroococcum nifUSVWZM gene cluster, including a new gene (nifP) which encodes a serine acetyl-transferase.J.Bacteriol. 173, 5457–5469 (1991).

    PubMed  CAS  Google Scholar 

  • Jacobson M.R., Cash V.L., Weiss M.C., Laird N.F., Newton W.E., Dean D.R.: Biochemical and genetic analysis of thenifUSVWZM cluster fromAzotobacter vinelandii.Mol.Gen.Genet. 219, 49–57 (1989).

    PubMed  Article  CAS  Google Scholar 

  • Masterson R.V., Prakash R.K., Atherly A.G.: Conservation of symbiotic nitrogen fixation gene sequences inRhizobium japonicum andBradyrhizobium japonicum.J.Bacteriol. 163, 21–26 (1985).

    PubMed  CAS  Google Scholar 

  • Mortenson L.E., Thorneley R.N.F.: Structure and function of nitrogenase.Ann.Rev.Biochem. 48, 387–418 (1979).

    PubMed  Article  CAS  Google Scholar 

  • Orosz L., Svab Z., Kondorosi A., Sik T.: Genetic studies onRhizophage 16-3. Genes and functions on the chromosome.Mol.Gen.Genet. 125, 341–350 (1973).

    PubMed  CAS  Google Scholar 

  • Paul W., Merrick M.: The nucleotide sequence of thenifM gene ofKlebsiella pneumoniae and identification of a newnif gene:nifZ.Eur.J.Biochem. 170, 259–265 (1987).

    PubMed  Article  CAS  Google Scholar 

  • Paul W., Merrick M.: The roles of thenifW, nifZ andnifM genes ofKlebsiella pneumoniae in nitrogenase biosynthesis.Eur.J.Biochem. 178, 675–682 (1989).

    PubMed  Article  CAS  Google Scholar 

  • Petrova N., Gigova L., Venkov P.: NifH and NifM proteins interact as demonstrated by the yeast two-hybrid system.Biochem. Biophys.Res.Commun. 270, 863–867 (2000).

    PubMed  Article  CAS  Google Scholar 

  • Petrova N., Gigova L., Venkov P.: Dimerization ofRhizobium meliloti NifH protein inSaccharomyces cerevisiae cells requires simultaneous expression of NifM protein.Internat.J.Biochem.Cell Biol. 34, 33–42 (2002).

    Article  CAS  Google Scholar 

  • Sambrook J., Fritsch E.F., Maniatis T.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 1989.

    Google Scholar 

  • Selenska-Trajkova S., Radeva G., Markov K.: Comparison betweenRhizobium galegae andRhizobium meliloti plasmid contents.Lett.Appl.Microbiol. 10, 123–126 (1990).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Academician M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    L. Gigova & S. Dimova

  2. Institute of Molecular Biology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    N. Petrova & D. Staneva

Authors
  1. L. Gigova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Petrova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Dimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Staneva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. Gigova.

Additional information

This work was supported byBulgarian Academy of Sciences.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gigova, L., Petrova, N., Dimova, S. et al. Existence ofSinorhizobium meliloti genomic fragment hybridizing with thenifM gene ofKlebsiella pneumoniae . Folia Microbiol 52, 447–448 (2007). https://doi.org/10.1007/BF02932102

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932102

Keywords

  • Nitrogen Fixation
  • Klebsiella Pneumoniae
  • Biological Nitrogen Fixation
  • nifH Gene
  • Bulgarian Academy