Folia Microbiologica

, 52:375 | Cite as

Ribotyping and biotyping ofStaphylococcus epidermidis isolated from hospital environment

  • L. Malíková
  • I. Sedláček
  • D. Nováková
  • M. Němec


TheStaphylococcus strains acquired from scrapings from hospital environments were identified to the species level based on their biochemical properties. From the monitored sample theStaphylococcus epidermidis strains were selected for more accurate typing and tested on their virulence factor and ribotyped. The biotyping ofS. epidermidis did not show any considerable intraspecific variation of these isolates and there were no atypical reactions, with the exception of three strains (out of 33). In contrast, the results of ribotyping showed greater heterogeneity of strains and unequivocally demonstrated the relation between the ribotype and the place of sample drawing. In addition to this fact, the found ribotypes repeat in the same environment in the long term which suggests the occurrence and persistence of the same strains of conditionally pathogenic bacteria in hospital environment. We showed that ribotyping is a suitable method for precise and reliable detection of some coagulase-negative staphylococci.


Linezolid Acetoin Hospital Environment Novobiocin Furazolidon 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Czech Collection of Microorganisms


Centre of Cardiovascular and Transplantation Surgery


coagulase-negative staphylococci


Masaryk Memorial Cancer Hospital


National Reference Laboratory


Traumatological Hospital


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • L. Malíková
    • 1
  • I. Sedláček
    • 2
  • D. Nováková
    • 2
  • M. Němec
    • 3
  1. 1.Regional Public Health InstituteBrnoCzechia
  2. 2.Czech Collection of Microorganisms, Faculty of ScienceMasaryk UniversityBrnoCzechia
  3. 3.Department of Microbiology, Faculty of ScienceMasaryk UniversityBrnoCzechia

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