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Synergistic utilization of dichloroethylene as sole carbon source by bacterial consortia isolated from contaminated sites in Africa

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Abstract

The widespread use and distribution of chloroethylene organic compounds is of serious concern owing to their carcinogenicity and toxicity to humans and wildlife. In an effort to develop active bacterial consortia that could be useful for bioremediation of chloroethylenecontaminated sites in Africa, 16 combinations of 5 dichloroethylene (DCE)-utilizing bacteria, isolated from South Africa and Nigeria, were assessed for their ability to degradecis- andtrans-DCEs as the sole carbon source. Three combinations of these isolates were able to remove up to 72% of the compounds within 7 days. Specific growth rate constants of the bacterial consortia ranged between 0.465 and 0.716 d−1 while the degradation rate constants ranged between 0.184 and 0.205 d−1, with 86.36–93.53 and 87.47–97.12% of the stoichiometric-expected chloride released during growth of the bacterial consortia, incis- andtrans-DCE, respectively. Succession studies of the individual isolates present in the consortium revealed that the biodegradation process was initially dominated byAchromobacter xylosoxidans and subsequently byAcinetobacter sp. andBacillus sp., respectively. The results of this study suggest that consortia of bacteria are more efficient than monocultures in the aerobic biodegradation of DCEs, degrading the compounds to levels that are up to 60% below the maximum allowable limits in drinking water.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science and Agriculture, University of KwaZulu-Natal, (Westville Campus), Private Bag X54001, 4000, Durban, Republic of South Africa

    Ademola O. Olaniran, Nokukhanya H. Mfumo, Dorsamy Pillay & Balakrishna Pillay

  2. Department of Biotechnology, Faculty of Engineering, Science and Built Environment, Durban Institute of Technology, P. O. Box 1334, 4000, Durban, Republic of South Africa

    Dorsamy Pillay

  3. Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ille-Ife, Nigeria

    Ademola O. Olaniran

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  1. Ademola O. Olaniran
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Correspondence to Ademola O. Olaniran.

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Olaniran, A.O., Mfumo, N.H., Pillay, D. et al. Synergistic utilization of dichloroethylene as sole carbon source by bacterial consortia isolated from contaminated sites in Africa. Biotechnol. Bioprocess Eng. 11, 205–210 (2006). https://doi.org/10.1007/BF02932031

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