Biotechnology and Bioprocess Engineering

, Volume 5, Issue 6, pp 441–448 | Cite as

Operation and modeling of bench-scale SBR for simultaneous removal of nitrogen and phosphorus using real wastewater

  • Seong-Jin Lim
  • Ra Kyung Moon
  • Woo Gi Lee
  • Sunhoon Kwon
  • Byung Geon Park
  • Ho Nam Chang


Experimental work was carried out on nitrogen and phosphorus removal from real wastewater using a bench-scale SBR process. The phosphorus removal was stable and the phosphorus concentration remaining in the reactor was maintained within 1.5 ppm, regard-less of the addition of an external carbon source. In the case of nitrogen, an external carbon source was necessary for denitrification. The effect on denitrification with the addition of various carbon sources, such as glucose, methanol, acetate, and propionate, was also investigated. Acetate was found to be the most effective among those tested in this study. When 100 ppm (theoretical oxygen demand) of sodium acetate was added, the average rate of denitrifiaction was 2.73 mg NO 3 -N (g MLSS)−1 h−1, which wasca. 4 times higher than that with the addition of 200 ppm of methanol. The phosphorus and nitrogen concentrations were both maintained within 1.5 ppm by the addition of an appropriate amount of a carbon source during a long-term operation of the SBR. The mathematical modeling was performed using Monod kinetics, other microbial kinetics, mass balances, and stoichiometry. The modeling was found to be useful for predicting the SBR operation and optimizing the HRT.


SBR nitrogen removal phosphorus removal wastewater treatment modeling 





Oxidized nitrogen








Readily biodegradable substrate


Slowly biodegradable substrate




Total Nitrogen


Total Phosphorus


Reactor working volume


Phosphorus removing microorganisms


Nitrifying microorganisms


Denitrifying microorganisms


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2000

Authors and Affiliations

  • Seong-Jin Lim
    • 1
  • Ra Kyung Moon
    • 1
  • Woo Gi Lee
    • 1
  • Sunhoon Kwon
    • 1
  • Byung Geon Park
    • 2
  • Ho Nam Chang
    • 1
  1. 1.Department of Chemical EngineeringKorea Advanced Institute of Science and TechnologyTaejonKorea
  2. 2.Department of Environmental and HealthKwang-ju Women's UniversityKwang-juKorea

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