Enhanced clavulanic acid production inStreptomyces clavuligerus NRRL3585 by overexpression of regulatory genes

  • Trinh Viet Hung
  • Kenji Ishida
  • Niranjan Parajuli
  • Kwangkyoung Liou
  • Hei Chan Lee
  • Jae Kyung Sohng


We constructed four recombinant plasmids to enhance the production of clavulanic acid (CA) inStreptomyces clavuligerus NRRL3585: (1) plBRHL1, which includesccaR, a pathway-specific regulatory gene involved in cephamycin C and CA biosynthesis; (2) plBRHL2, containingclaR, again a regulatory gene, which controls the late steps of CA biosynthesis; (3) pGIBR containingafsR-p, a global regulatory gene fromStreptomyces peucetius, and (4) pKS, which harbors all of the genes (ccaR/claR/afsR-p). The plasmids were expressed inS. clavuligerus NRRL3585 along with theermE * promoter. All of them enhanced the production of CA; 2.5-fold overproduction for plBRHL1, 1.5-fold for plBRHL2, 1.6-fold for pGIBR, and 1.5-fold for pKS compared to the wild type.


clavulanic acid production Streptomyces clavuligerus regulatory gene ccaR claR afsR-p 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2006

Authors and Affiliations

  • Trinh Viet Hung
    • 1
  • Kenji Ishida
    • 1
  • Niranjan Parajuli
    • 1
  • Kwangkyoung Liou
    • 1
  • Hei Chan Lee
    • 1
  • Jae Kyung Sohng
    • 1
  1. 1.Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical EngineeringSun Moon UniversityAsanKorea

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