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Production of vanillin from ferulic acid using recombinant strains ofEscherichia coli

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Abstract

Vanillin is one of the world's principal flavoring compounds, and is used extensively in the food industry. The potential vanillin production of the bacteria was compared to select and clone genes which were appropriate for highly productive vanillin production byE. coli. Thefcs (feruloyl-CoA synthetase) andech (enoyl-CoA hydratase/aldolase) genes cloned fromAmycolatopsis sp. strain HR104 andDelftia acidovorans were introduced to pBAD24 vector with PBAD promoter and were named pDAHEF and pDDAEF, respectively. We observed 160 mg/L vanillin production withE. coli harboring pDAHEF, whereas 10 mg/L of vanillin was observed with pDDAEF. Vanillin production was optimized withE. coli harboring pDAHEF. Induction of thefcs andech genes from pDAHEF was optimized with the addition of 13.3 mM arabinose at 18 h of culture, from which 450 mg/L of vanillin was produced. The feeding time and concentration of ferulic acid were also optimized by the supplementation of 0.2% ferulic acid at 18 h of culture, from which 500 mg/L of vanillin was obtained. Under the above optimized condition of arabinose induction and ferulic acid supplementation, vanillin production was carried out with four different types of media, M9, LB, 2YT, and TB. The highest vanillin production, 580 mg/L, was obtained with LB medium, a 3.6 fold increase in comparison to the 160 mg/L obtained before the optimization of vanillin production.

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Authors and Affiliations

  1. Department of Food Science & Nutrition, Gyeongsang National University, 660-701, Jinju, Korea

    Sang-Hwal Yoon, Cui Li, Young-Mi Lee, Sung-Hee Kim & Seon-Won Kim

  2. Division of Applied Life Science (BK21), Gyeongsang National University, 660-701, Jinju, Korea

    Sook-Hee Lee, Jae-Yeon Kim & Seon-Won Kim

  3. Division of Forest Science, Gyeongsang National University, 660-701, Jinju, Korea

    Myung-Suk Choi & Jae-Kyung Yang

  4. Department of Food Science, Jinju National University, 660-758, Jinju, Korea

    Weon-Taek Seo

  5. Institute of Agriculture and Life Science, Gyeongsang National University, 660-701, Jinju, Korea

    Seon-Won Kim

  6. Environmental Biotechnology National Core Research Center, Gyeongsang National University, 660-701, Jinju, Korea

    Jae-Yeon Kim & Seon-Won Kim

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  1. Sang-Hwal Yoon
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Yoon, SH., Li, C., Lee, YM. et al. Production of vanillin from ferulic acid using recombinant strains ofEscherichia coli . Biotechnol. Bioprocess Eng. 10, 378–384 (2005). https://doi.org/10.1007/BF02931859

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