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Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph

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Abstract

The effect of silkworm hemolymph on the expression of recombinant protein inEscherichia coli was investigated. The addition of silkworm hemolymph to the culture medium increased the production of recombinant β-galactosidase inE. coli. The production was dependent on the concentration of the added silkworm hemolymph, which increased 2-, 5-, and 8-fold in media supplemented with 1,3, and 5% silkworm hemolymph, respectively. To identify the effective component, the silkworm hemolymph was fractionated by gel filtration column chromatography. A fraction, with a molecular weight of about 30 K was identified as the effective component.

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Authors and Affiliations

  1. School of Chemical and Biological Engineering, Seoul National University, 151-744, Seoul, Korea

    Ji Eun Kim, Eun Jeong Kim, Won Jong Rhee & Tai Hyun Park

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  1. Ji Eun Kim
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  2. Eun Jeong Kim
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  3. Won Jong Rhee
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  4. Tai Hyun Park
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Correspondence to Tai Hyun Park.

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Kim, J.E., Kim, E.J., Rhee, W.J. et al. Enhanced production of recombinant protein inEscherichia coli using silkworm hemolymph. Biotechnol. Bioprocess Eng. 10, 353–356 (2005). https://doi.org/10.1007/BF02931854

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  • DOI: https://doi.org/10.1007/BF02931854

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