Three strains of rumen treponemes were isolated and partially characterized. The strains differed significantly one from another in morphology, fermentation characteristics and plasmid profiles. Their genetic variability was assayed using DNA-based molecular approaches. Easily differentiated ARDRA (amplified ribosomal DNA restriction analysis) patterns indicated that the strains represent different bacterial species.
KeywordsFructan Rumen Fluid Plasmid Profile Bovine Rumen Slovak Grant Agency Vega
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- Hungate R.E.: A roll tube method for cultivation of strict anaerobes, pp. 47–132 in J.R. Norris; D.W. Ribbons (Eds):Methods in Microbiology, Vol. 4. Academic Press, New York 1969.Google Scholar
- Paster B.J., Canale-Parola E.:Treponema saccharophilum sp.nov., a large pectinolytic spirochete from the bovine rumen.Appl.Enviorn.Microbiol. 50, 212–219 (1985).Google Scholar