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Chitosanase activity inBacillus thuringiensis

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Abstract

The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23 %) out of 77 crystalliferous strains ofBacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 °C with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase ofB. thuringiensis.

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Authors and Affiliations

  1. Departamento de Microbiologia, Escuela Nacional de Ciencias Biológicas, Instituto Politecnico National, 11340 (DF), México, México

    R. Cruz Camarillo, O. Sánchez Pérez, M. Gómez Ramírez & L. I. Rojas Avelizapa

  2. Programa de Biotecnología, Instituto Mexicano del Petróleo, 07730 (DF), México, México

    N. G. Rojas Avelizapa

Authors
  1. R. Cruz Camarillo
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  2. O. Sánchez Pérez
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  3. N. G. Rojas Avelizapa
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  4. M. Gómez Ramírez
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  5. L. I. Rojas Avelizapa
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Corresponding author

Correspondence to R. Cruz Camarillo.

Additional information

This work was partially supported by Consejo Nacional de Ciencia y Tecnologia (CONACYT) México (project J-34997-B); by theCoordinación General de Estudios de Posgradoe Investigación (CGEPI)Instituto Politecnico National (IPN) (project 980 434), and by theComisión de Operación y Fomento de Actividades Académicas (COFAA) IPN.

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Cruz Camarillo, R., Sánchez Pérez, O., Rojas Avelizapa, N.G. et al. Chitosanase activity inBacillus thuringiensis . Folia Microbiol 49, 94–96 (2004). https://doi.org/10.1007/BF02931653

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  • DOI: https://doi.org/10.1007/BF02931653

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