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Folia Microbiologica

, 49:94 | Cite as

Chitosanase activity inBacillus thuringiensis

  • R. Cruz Camarillo
  • O. Sánchez Pérez
  • N. G. Rojas Avelizapa
  • M. Gómez Ramírez
  • L. I. Rojas Avelizapa
Article

Abstract

The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23 %) out of 77 crystalliferous strains ofBacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 °C with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase ofB. thuringiensis.

Keywords

Chitosan Chitin Chitinase Bacillus Thuringiensis Serratia Marcescens 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2004

Authors and Affiliations

  • R. Cruz Camarillo
    • 1
  • O. Sánchez Pérez
    • 1
  • N. G. Rojas Avelizapa
    • 2
  • M. Gómez Ramírez
    • 1
  • L. I. Rojas Avelizapa
    • 1
  1. 1.Departamento de Microbiologia, Escuela Nacional de Ciencias BiológicasInstituto Politecnico NationalMéxicoMéxico
  2. 2.Programa de BiotecnologíaInstituto Mexicano del PetróleoMéxicoMéxico

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