Folia Microbiologica

, 49:94 | Cite as

Chitosanase activity inBacillus thuringiensis

  • R. Cruz Camarillo
  • O. Sánchez Pérez
  • N. G. Rojas Avelizapa
  • M. Gómez Ramírez
  • L. I. Rojas Avelizapa


The ability to produce extracellular chitosanase (EC was found by plate assays in 18 (23 %) out of 77 crystalliferous strains ofBacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 °C with shaking in a medium of pH 6.5 with 4 % colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase ofB. thuringiensis.


Chitosan Chitin Chitinase Bacillus Thuringiensis Serratia Marcescens 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Chavez Camarillo G., Cruz Camarillo R.: The chitinolytic system ofSerratia marcescens. (In Spanish)Rev.Lat.Amer.Microbiol. 26, 203–215 (1984).Google Scholar
  2. Davis B., Eveleigh D.E.: Chitosanases: occurrence, production and immobilization, pp. 161–179 in J.P. Zikakis (Ed.):Chitin, Chitosan and Related Enzymes. Academic Press, Orlando (USA)-London 1984.Google Scholar
  3. Fenton D.M., Eveleigh D.E.: Purification and mode of action of a chitosanase fromPenicillium islandicum.J.Gen.Microbiol. 126, 151–165 (1981).Google Scholar
  4. Gómez Ramírez M., Rojas-Avelizapa L.I., Cruz Camarillo R.: The chitinase ofBacillus thuringiensis, pp. 273–282 in R.A.A. Muzzarelli (Ed.):Chitin Enzymology 2001. University of Ancona (Italy) 2001.Google Scholar
  5. Helgason E., Okstad O.A., Caugan D.A., Johansen H.A., Fou A., Mok M., Hegna I.:Bacillus anthracis, B. cereus, andB. thuringiensis. One species on the basis of genetic evidence.Appl.Environ.Microbiol. 66, 2627–2630 (2000).PubMedCrossRefGoogle Scholar
  6. Kopečný J., Hodrová B.: Chitinolytic enzymes produced by ovine rumen bacteria.Folia Microbiol. 45, 465–468 (2000).CrossRefGoogle Scholar
  7. Kurakake M., Yo-u Sh., Nakagawa K., Sugihara M., Komaki T.: Properties of chitosanase fromBacillus cereus S1.Curr.Microbiol. 40, 6–9 (2000).PubMedCrossRefGoogle Scholar
  8. Lambert B., Pferoen M.: Insecticidal promise ofBacillus thuringiensis — facts and mysteries about a successful biopesticide.BioScience 42, 112–122 (1992).CrossRefGoogle Scholar
  9. Li E., Yousten A.: Metalloprotease fromBacillus thuringiensis.Appl.Microbiol. 30, 354–361 (1975).PubMedGoogle Scholar
  10. Monaghan R.L., Eveleigh D.E., Tewary R.P., Reese E.T.: Chitosanase, a novel activity.Nature New Biol. 245, 78–80 (1973).PubMedGoogle Scholar
  11. Pelletier A., Sygush J.: Purification and characterization of three chitosanase activities fromBacillus megaterium PI.Appl.Environ. Microbiol. 56, 844–848 (1990).PubMedGoogle Scholar
  12. Priest F.G.: Extracellular enzyme synthesis in the genusBacillus.Bacteriol.Rev. 41, 711–753 (1977).PubMedGoogle Scholar
  13. Ribas L.A., Parra V., Moreno-Paz M., Mellado R.P.: TheBacillus subtilis 168csn gene encodes a chitosanase with similar properties to aStreptomyces enzyme.Microbiology 146, 2929–2936 (2000).Google Scholar
  14. Rojas Avelizapa L.I., Cruz Camarillo R., Guerrero M.I., Rodríguez Vázquez R., Ibarra J.E.: Selection and characterization of a proteo-chitinolytic strain ofBacillus thuringiensis, able to grow in shrimp waste media.World J.Microbiol.Biotechnol. 15, 299–308 (1999).CrossRefGoogle Scholar
  15. Rojas Avelizapa L.I., Gómez Ramírez M., Cruz Camarillo R.: Fermentation of shrimp waste withBacillus thuringiensis to produce proteo-chitinolytic enzymes and insecticidal crystals, pp. 479–487 in R.R.A. Muzarelli, C. Muzarelli (Eds):Chitosan in Pharmacy and Chemistry. ATEC Edizioni, Ancona (Italy) 2002.Google Scholar
  16. Shimosaka M., Nagawa M., Ohno Y., Okasaki M.: Chitosanase from the plant pathogenic fungus,Fusarium solani f.sp.phaseoli. Purification and some properties.Biosci.Biotechnol.Biochem. 57, 231–235 (1993).CrossRefGoogle Scholar
  17. Šimůnek J., Hodrová B., Bartoňová H., Kopečný J.: Chitinolytic bacteria of the mammal digestive tract.Folia Microbiol. 46, 76–78 (2001).CrossRefGoogle Scholar
  18. Šimůnek J., Kopečný J., Hodrová B., Bartoňová H.: Identification and characterization ofClostridium paraputrificum, a chitinolytic bacterium of human digestive tract.Folia Microbiol. 47, 559–564 (2002).CrossRefGoogle Scholar
  19. Somashekarar D., Joseph R.: Chitosanases. Properties and applications: a review.Biores.Technol. 55, 35–45 (1995).CrossRefGoogle Scholar
  20. Tsuru D., Yoshimoto T.: Microbial proteases, pp. 239–283 in A.I. Laskin, H.A. Lechevalier (Eds):CRC Handbook of Microbiology, Vol. 8. CRC Press, Boca Raton (USA) 1987.Google Scholar
  21. Uchida Y., Ohtakara A.: Chitosanase fromBacillus species.Meth.Enzymol. 161, 501–505 (1988).CrossRefGoogle Scholar
  22. Yabuki M., Uchiyama A., Suzuki A., Ando A., Fuji T.: Purification and properties of chitosanase fromBacillus circulans MH-KI.J.Gen.Appl.Microbiol. 34, 255–270 (1988).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2004

Authors and Affiliations

  • R. Cruz Camarillo
    • 1
  • O. Sánchez Pérez
    • 1
  • N. G. Rojas Avelizapa
    • 2
  • M. Gómez Ramírez
    • 1
  • L. I. Rojas Avelizapa
    • 1
  1. 1.Departamento de Microbiologia, Escuela Nacional de Ciencias BiológicasInstituto Politecnico NationalMéxicoMéxico
  2. 2.Programa de BiotecnologíaInstituto Mexicano del PetróleoMéxicoMéxico

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