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Folia Microbiologica

, Volume 51, Issue 6, pp 609–613 | Cite as

Prevention of infection transmission during stem cell transplantation

  • M. Bláha
  • P. Měřička
  • V. Štěpánová
  • M. Špliňo
  • J. Malý
  • L. Jebavý
  • P. Žák
  • M. Cermanová
  • S. Filip
  • M. Blažek
  • V. Řeháček
Article

Abstract

Group of 152 patients (investigated before autologous transplantation) and 35 healthy donors for allogeneic transplantation was examined for the risk of infection transmission that can be associated with the infusion of cryopreserved peripheral blood progenitor cells to the patient and/or cross-contamination of stored grafts. No laboratory signs of active infection were found in 22 donors (63 %) and in 91 patients (60%). The most common was active infection by herpes viruses — 50 cases in patients, 21 cases in donors; hepatitis B was found in only two cases. The rate of clinically unsuspected (but dangerous) infections in donors and patients thus remains relatively high in spite of the fact that the system of donor search and the whole transplantation procedure have improved in the last years. The system of safety assurance is extremely important and the whole palette of preventive tests according to EBMT (European Blood and Marrow Transplantation Group) and ISHAGE (International Society for Hemotherapy and Graft Engineering) is fully justified.

Keywords

Hematopoietic Stem Cell Transplantation Toxoplasmosis Infection Transmission Allogeneic Transplantation Peripheral Stem Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AU

arbitrary unit

EIA

enzyme immunoassay method

EBMT

European Blood and Marrow Transplantation Group

HR

International Institute of Refrigeration

ISHAGE

International Society for Hemotherapy and Graft Engineering

CMV

cytomegalovirus

EBV

Epstein-Barr virus

HAV

hepatitis A virus

HIV

human immunodeficiency virus

G-CSF

granulocyte colony-stimulating factor

SCT

stem cell transplantation(s)

HSV

herpes simplex virus

HTLV

human T-cell lymphotropic virus

VZV

varicella-zoster virus

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References

  1. Blaha M., Skořepová M., Filip S., Maisnar V., Voglova J., Žák P., Kmoníček M., Malý J., Měřička P.: Increasing yields in peripheral stem cell separations for transplantation, p. 177 inESFH (Eds):Proc. 13th Congr. European Soc. Hemapheresis, Riva del Garda (Italy) 2001.Google Scholar
  2. Bláha M., Měřička P., Štěpánová V., Maly J., Vávra L., Jebavý L.: Potential risk of infection transmission during storage and transplantation of hematological progenitor cells.Folia Microbiol. 48, 399–402 (2003).CrossRefGoogle Scholar
  3. Gratwohl A., Baldomero H., Schmid O., Horisberger B., Bargetzi M., Urbano-Ispizua A.: Change in stem cell source for hematopoietic stem cell transplantation (HSCT) in Europe: a report of the EBMT activity survey 2003.Bone Marrow Transplant. 36, 575–590 (2005a).CrossRefPubMedGoogle Scholar
  4. Gratwohl A., Brand R., Frassoni F., Rocha V., Niederwieser D., Reusser P., Einsele H., Cordonnier C.: Cause of death after allogeneic hematopoietic stem cell transplantation (HSCT) in early leukemias: an EBMT analysis of lethal infectious complications and changes over calendar time.Bone Marrow Transplant.36, 757–769 (2005b).CrossRefPubMedGoogle Scholar
  5. Hawkins A.E., Zuckerman M.A., Briggs M., Gilson R.J.C., Goldstone A.H., Brink N.S., Tedder R.S.: Hepatitis B nucleotide sequence analysis, linking an outbreak of acute hepatitis B to contamination of a cryopreservation tank.J.Virol.Meth. 60, 81–88 (1996).CrossRefGoogle Scholar
  6. Hitoshi O.: Efficient and safe stem cell apheresis.Transf.Sci. 31, 203–205 (2004).Google Scholar
  7. Kaiser R., Geulen O., Matz B.: Risk of hepatitis C after immunoadsorption.Infect.Control Hospital Epidemiol. 23, 342–343 (2002).CrossRefGoogle Scholar
  8. Khu H.M., Cowley H., David-Ocampo V., Carter C.S., Kasten-Sportes C., Wayne A.S., Solomon S.R., Bishop M.R., Childs R.M., Read E.J.: Catastrophic failures of freezing bags for cellular therapy products: description, cause, and consequences.Cytotherapy 4, 539–549 (2002).CrossRefGoogle Scholar
  9. Lin D.B., Su K.E., Yu J.C.: Studies on cryopreservation ofToxoplasma gondii and its antigenicity in mice.Am.J.Trop.Med.Hyg. 53, 392–396 (1995).PubMedGoogle Scholar
  10. Měřička P.: Cryopreservation of hematopoietic progenitor cells for autologous and allogeneic transplantation, p. 773 inIIR (Eds):Proc. 2nd Internat. Congr. Refrigeration, Sydney (Australia) 1999.Google Scholar
  11. Měřička P.: Contribution to the safe keeping of cells and tissues at −80 °C, p. 217 inIIR (Eds):Cryogenics 2000, 6th Cryogenics IIR Internat. Conf., Prague 2000.Google Scholar
  12. Meřička P., Blaha M., Vávra L., Štěpánova V.: Our system of cross-contamination prevention during storage of hematopoietic progenitor cells, p. 302 in G. Grof, M. Menzer (Eds):Proc. 21st IIR Internat. Congr. Refrigeration, Washington (DC) 2003.Google Scholar
  13. Rajčáni J., Ďurmanová V.: Early expression of herpes simplex virus proteins and reactivation of latent infection.Folia Microbiol. 45, 7–28 (2000).CrossRefGoogle Scholar
  14. Tedder R.S., Zuckerman M.A., Goldstone A.H., Hawkins A.E., Fielding A., Briggs E.M., Irwin D., Blair S., Gorman A.M., Patterson K.G., Linch D.C., Heptstonstall J., Brinsks N.S.: Hepatitis B transmission from contaminated cryopreservation tank.Lancet 346, 137–140 (1995).CrossRefPubMedGoogle Scholar
  15. Warkentin P.I.: Cryopreservation and infusion of peripheral stem cells, pp. 199–205 in W.W. Wunder, P.R. Henon (Eds):Peripheral Stem Cell Autografts. Springer-Verlag, Heidelberg 1993.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2006

Authors and Affiliations

  • M. Bláha
    • 1
  • P. Měřička
    • 1
  • V. Štěpánová
    • 1
  • M. Špliňo
    • 1
  • J. Malý
    • 1
  • L. Jebavý
    • 1
  • P. Žák
    • 1
  • M. Cermanová
    • 1
  • S. Filip
    • 1
  • M. Blažek
    • 1
  • V. Řeháček
    • 1
  1. 1.University Hospital, Medical FacultyCharles UniversityHradec KrálovéCzechia

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