Folia Microbiologica

, 50:415 | Cite as

Agrobacterium tumefaciens-mediated transformation ofMucor circinelloides

  • I. Nyilasi
  • K. Ács
  • T. Papp
  • E. Nagy
  • C. Vágvölgyi


TheAgrobacterium tumefaciens-mediated transformation of the zygomycetous fungusMucor circinelloides is described. A method was also developed for the hygromycin B-based selection ofMucor transformants. Transformation with the hygromycin B phosphotransferase gene ofEscherichia coli controlled by the heterologousAspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.


Hygromycin Induction Medium Crown Gall Rose Bengal Putative Transformants 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  • I. Nyilasi
    • 1
  • K. Ács
    • 2
  • T. Papp
    • 2
  • E. Nagy
    • 1
  • C. Vágvölgyi
    • 2
  1. 1.HAS-USZ Microbiological Research Group, Department of MicrobiologyUniversity of SzegedSzegedHungary
  2. 2.Department of Microbiology, Faculty of ScienceUniversity of SzegedSzegedHungary

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