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Molecular analysis of 16S–23S spacer regions ofAcetobacter species

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Abstract

16S–23S rDNA internal transcribed spacer regions (ITS) similarities were determined in 8Acetobacter and 1Gluconacetobacter strains. ITS-PCR amplification of the 16S–23S spacers showed 2 products of similar size in 7 strains; only 1 product of similar size was found in the 2 remaining strains. Analysis of the PCR products using restriction endonucleasesHaeIII,HpaII andAluI revealed 3 different restriction groups ofA. pasteurianus forAluI andHaeIII, and 4 restriction groups forHpaII. ITS nucleotide sequences of all studied strains exhibited a 52–98% similarity.

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Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Science, Comenius University, 842 15, Bratislava, Slovakia

    M. Kretová & J. Grones

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  1. M. Kretová
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  2. J. Grones
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Correspondence to J. Grones.

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This work was supported by theSlovak Grant Agency VEGA (grant 1/7230/20) andComenius University (grant 66/2002-UK).

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Kretová, M., Grones, J. Molecular analysis of 16S–23S spacer regions ofAcetobacter species. Folia Microbiol 50, 288–292 (2005). https://doi.org/10.1007/BF02931408

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