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Folia Microbiologica

, Volume 50, Issue 4, pp 288–292 | Cite as

Molecular analysis of 16S–23S spacer regions ofAcetobacter species

  • M. Kretová
  • J. Grones
Article

Abstract

16S–23S rDNA internal transcribed spacer regions (ITS) similarities were determined in 8Acetobacter and 1Gluconacetobacter strains. ITS-PCR amplification of the 16S–23S spacers showed 2 products of similar size in 7 strains; only 1 product of similar size was found in the 2 remaining strains. Analysis of the PCR products using restriction endonucleasesHaeIII,HpaII andAluI revealed 3 different restriction groups ofA. pasteurianus forAluI andHaeIII, and 4 restriction groups forHpaII. ITS nucleotide sequences of all studied strains exhibited a 52–98% similarity.

Keywords

Internal Transcribe Space Space Region Restriction Group Acetic Acid Bacterium Vinegar Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  1. 1.Department of Molecular Biology, Faculty of ScienceComenius UniversityBratislavaSlovakia

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