Biotechnology and Bioprocess Engineering

, Volume 12, Issue 5, pp 525–530 | Cite as

Effect of mobile phase additives on resolution of some nucleic compounds in high performance liquid chromatography

  • Chun Hua Jin
  • Yoon Mo Koo
  • Dae-Ki Choi
  • Kyung Ho Row


Here we investigate the chromatographic behavior, with reversed-phase high performance liquid chromatography (RP-HPLC) of nucleic compounds (nucleobases, nucleosides, and nucleotides) on a C18 column in several different mobile phase additives, including1-butyl-3-methylimidazolium tetrafuloroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]) ionic liquids, ammonium formate, and potassium phosphate. The effect of the alkyl group length, the imidazolium ring, and the ionic liquid's counterions on retention and resolution of the samples were tested. The results show the potential application of a used buffer system, ion pairing system, and ionic liquid as mobile phase additives in liquid chromatography resolution of nucleic compounds.


ionic liquid buffer ion pairing RP-HPLC nucleic compounds 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2007

Authors and Affiliations

  • Chun Hua Jin
    • 1
  • Yoon Mo Koo
    • 1
  • Dae-Ki Choi
    • 2
  • Kyung Ho Row
    • 1
  1. 1.Center for Advanced Bioseparation Technology and Department of Chemical EngineeringInha UniversityIncheonKorea
  2. 2.Clean and Technology Research CenterKorea Institute of Science and TechnologySeoulKorea

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