Folia Microbiologica

, Volume 48, Issue 4, pp 521–524 | Cite as

Detection ofBurkholderia pseudomallei in rice fields with PCR-based technique

  • C. M. Kao
  • S. C. Chen
  • Y. S. Chen
  • H. M. Lin
  • Y. L. Chen


Burkholderia pseudomallei Ara in rice fields was detected using PCR-based techniques with 16S RNA and flagella gene primer sets. The sensitivity of these PCRs was at least 1 CFU/mL ofB. pseudomallei Ara preincubated into Ashdown’s medium for 6 h.B. pseudomallei Ara DNA from watery soil were more detectable than from dry soil. The distribution of this DNA was mainly found at a depth of 300–600 mm under crop-covered fields, but not detected in the location of soil close to the land surface. The results suggest that PCR based on 16S RNA and flagella gene primer sets can be applied to investigate the presence ofB. pseudomallei Ara in contaminated soil of rice fields.


Rice Field Melioidosis Burkholderia Pseudomallei Flagellin Gene Flagellum Gene 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2003

Authors and Affiliations

  • C. M. Kao
    • 1
  • S. C. Chen
    • 2
  • Y. S. Chen
    • 3
    • 4
  • H. M. Lin
    • 5
  • Y. L. Chen
    • 5
  1. 1.Institute of Environmental EngineeringNational Sun Yat-Sen UniversityKaohsiungTaiwan
  2. 2.Department of MedicineChina Medical CollegeTaichungTaiwan
  3. 3.Section of Infectious DiseasesVeterans General Hospital-KaohsiungKaohsiungTaiwan
  4. 4.Institute of Biomedical SciencesNational Sun Yat-Sen UniversityKaohsiungTaiwan
  5. 5.Department of Medical TechnologyFooyin UniversityTa-Liao. Kaohsiung HsienTaiwan

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