Abstract
Burkholderia pseudomallei Ara− in rice fields was detected using PCR-based techniques with 16S RNA and flagella gene primer sets. The sensitivity of these PCRs was at least 1 CFU/mL ofB. pseudomallei Ara− preincubated into Ashdown’s medium for 6 h.B. pseudomallei Ara− DNA from watery soil were more detectable than from dry soil. The distribution of this DNA was mainly found at a depth of 300–600 mm under crop-covered fields, but not detected in the location of soil close to the land surface. The results suggest that PCR based on 16S RNA and flagella gene primer sets can be applied to investigate the presence ofB. pseudomallei Ara− in contaminated soil of rice fields.
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References
Brett P.J., DeShazer D., Woods D.E.:Burkholderia thailandensis sp.nov., aBurkholderia pseudomallei-like species.Internat.J.Syst.Bacteriol.48, 317–320 (1998).
Brook M.D., Currie B., Desmarchelier P.M.: Isolation and identification ofBurkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction.J.Appl.Microbiol.82, 589–596 (1997).
Chaowagul W., White N.J., Dance D.A., Wattanagoon Y., Naigowit P., Davis T.M., Looareesuwan S., Pitakwatchara N.: Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand.J.Infect.Dis.159, 890–899 (1989).
Chen Y.S., Chen S.C., Kao C.M., Chen Y.L.: Effects of pH, temperature and water contents on the growth ofBurkholderia pseudomallei in the soil.Folia Microbiol.48, 253–256 (2003).
Dance D.A.: Melioidosis: the tip of the iceberg?Clin.Microbiol.Rev.4, 52–60 (1991).
Dance D.A.: Ecology ofBurkholderia pseudomallei and the interactions between environmentalBurkholderia spp. and human-animal hosts.Acta Trop.74, 159–168 (2000).
Dharakul T., Tassaneetrithep B., Trakulsomboon S., Songsivilai S.: Phylogenetic analysis of Ara+ and Ara−Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes.J.Clin.Microbiol.37, 1906–1912 (1999).
Leelarasamee A., Bovornkitti S.: Melioidosis: review and update.Rev.Infect.Dis.11, 413–425 (1989).
Merianos A., Patel M., Lane J.M., Noonan C., Sharrock D., Mock P.A., Currie B.: The 1990–1991 outbreak of melioidosis in the Northern Territory of Australia: epidemiology and environmental studies.Southeast Asian J.Trop.Med.Public Health24, 425–435 (1993).
Smith M.D., Angus B.J., Wuthiekanun V., White N.J.: Arabinose assimilation defines a nonvirulent biotype ofBurkholderia pseudomallei.Infect.Immun.65, 4319–4321 (1997).
Sonthayanon P., Krasao P., Wuthiekanun V., Panyim S., Tungpradabkul S.: A simple method to detect and differentiateBurkholderia pseudomallei andBurkholderia thailandensis using specific flagellin gene primers.Mol.Cell Probes16, 217–222 (2002).
Suputtamongkol Y., Hall A.J., Dance D.A., Chaowagul W., Rajchanuvong A., Smith M.D., White N.J.: The epidemiology of melioidosis in Uben Ratchatani, northeast Thailand.Internat.J.Epidemiol.23, 1082–1090 (1994).
Thomas A.D., Forbes-Faulkner J.C.: Persistence ofPseudomonas pseudomallei in soil.Austral.Vet.J.57, 535–536 (1981).
Trakulsomboon S., Vuddhakul V., Tharavichitkul P., Na-Gnam N., Suputtamongkol Y., Thamlikitkul V.: Epidemiology of arabinose assimilation inBurkholderia pseudomallei isolated from patients and soil in Thailand.Southeast Asian J.Trop.Med.Public Health30, 756–759 (1999).
Vuddhakul V., Tharavichitkul P., Na-Ngam N., Jitsurong S., Kunthawa B., Noimay P., Noimay P., Binla A., Thamlikitkul V.: Epidemiology ofBurkholderia pseudomallei in Thailand.Am.J.Trop.Med.Hyg.60, 458–461 (1999).
Wajanarogana S., Sonthayanon P., Wuthiekanun V., Panyim S., Simpson A.J., Tungpradabkul S.: Stable marker on flagellin gene sequence related to arabinose non-assimilating pathogenicBurkholderia pseudomallei.Microbiol.Immunol.43, 995–1001 (1999).
Wuthiekanun V., Dance D.A., Wattanagoon Y., Supputtamongkol Y., Chaowagul W., White N.J.: The use of selective media for the isolation ofPseudomonas pseudomallei in clinical practice.J.Med.Microbiol.33, 121–126 (1990).
Wuthiekanum V., Smith M. D., Dance D.A., White N.J.: Isolation ofPseudomonas pseudomallei from soil in northeastern Thailand.Trans.Roy.Soc.Trop.Med.Hyg.89, 41–43 (1995).
Wuthiekanum V., Smith M.D., Dance D.A., Walsh A.L., Pitt T.L., White N.J.: Biochemical characteristics of clinical and environmental isolates ofBurkholderia pseudomallei.J.Med.Microbiol.45, 408–412 (1996).
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This project was funded in part byNational Science Council, Republic of China (no. 90-2626-B-242-001).
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Kao, C.M., Chen, S.C., Chen, Y.S. et al. Detection ofBurkholderia pseudomallei in rice fields with PCR-based technique. Folia Microbiol 48, 521–524 (2003). https://doi.org/10.1007/BF02931334
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DOI: https://doi.org/10.1007/BF02931334