Summary
Changes in pancreatic exocrine functions were compared between conscious rats with bile duct ligation and bile diversion from the duodenum on the first, third, fifth, and seventh postoperative days. Body weight was significantly decreased with time in both groups. Basal secretions of fluid, bicarbonate, and protein remained unchanged throughout the experimental period in bile duct ligated rats, whereas in bile diverted rats, the basal bicarbonate secretion with returning of pancreatic juice to the duodenum increased on the third postoperative day, and the basal protein output significantly increased with time. Basal secretions with returning of pancreatic juice to the duodenum in both groups were higher than that in control (bile and pancreatic juice returned to the intestine) rats. Stepwise increases in fluid and bicarbonate outputs responding to the graded doses of secretin were observed in bile duct ligated rats on the first and third postoperative days, as has been observed in bile diverted rats. However, on the fifth and seventh postoperative days, stepwise responses to graded doses of secretin were no longer observed in bile duct ligated rats. The pancreatic response to cerulein was greater in bile diverted rats than in bile duct ligated rats. Plasma CCK concentration in 7-d bile duct ligated rats (4.7 pM) was significantly higher than that in 7-d bile diverted rats (1.6 pM), although the pancreatic wet weight, protein concentration, and total content were comparable for the two groups. It was suggested that the presence of bile in the duodenum is required to maintain normal pancreatic secretion, and that the removal of bile from the intestine has quite different effects, depending on whether the bile flow is obstructed or diverted.
Similar content being viewed by others
References
Green GM, Nasset ES. Effect of bile duct obstruction on pancreatic enzyme secretion and intestinal proteolytic enzyme activity in the rat. Dig. Dis. 1977; 22: 437–443.
Green GM, Nasset ES. Importance of bile in regulation of intraluminal proteolytic enzyme activities in the rat. Gastroenterology 1980; 79: 695–702.
Green GM, Lyman RL. Feedback regulation of pancreatic enzyme secretion as a mechanism for trypsin inhibitor-induced hypersécrétion in rats. Proc. Soc. Exp. Biol. Med. 1972; 140: 6–12.
Green GM, Olds BA, Matthews G, Lyman RL. Protein as a regulator of pancreatic enzyme secretion in the rat. Proc. Soc. Exp. Biol. Med. 1973; 142: 1162–1167.
Miyasaka K, Green GM. Effect of atropine on rat basal pancreatic secretion during return or diversion of bile-pancreatic juice. Proc. Soc. Exp. Biol. Med. 1983; 174: 187–192.
Keller PJ, Cohen E, Neurath H. The proteins of bovine pancreatic juice. J. Biol. Chem. 1958; 233: 344–349.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265–275.
Liddle RA, Goldfine ID, Williams JA. Bioassay of plasma cholecystokinin in rats: effects of food, trypsin inhibitor, and alcohol. Gastroenterology 1984; 87: 542–549.
Louie DS, May D, Miller P, Owyang C. Cholecystokinin mediates feedback regulation of pancreatic enzyme secretion in rats. Am. J. Physiol. 1986; 250: G252-G259.
Hirata K, Maruyama Y, Shiramatsu K, Hayasaka H. Analysis of the acceleration of the exocrine pancreatic function under the obstructive jaundice in rat. Jap. J. Surg. Metab. Nutr. 1982; 16: 552–559.
Baba N, Suzuki T, Tobe T, Inoue K, Chowdhury P, Chang LW, Rayford PL. Influence of obstructive jaundice on pancreatic growth and on basal plasma levels of cholecystokinin and gastrin in rats. Dig. Dis. Sci. 1986; 31: 1233–1241.
Schneeman BO, Lyman RL. Factors involved in the intestinal feedback regulation of pancreatic enzyme secretion in the rat. Proc. Soc. Exp. Biol. Med. 1975; 148: 897–903.
Brand SJ, Morgan RGH. The release of rat intestinal cholecystokinin after oral trypsin inhibitor measured by bio-assay. J. Physiol. London 1981; 319: 325–343.
Fölsch UR, Cantor P, Wilms HM, Shafmayer A, Becker HD, Creutzfeldt W. Role of cholecystokinin in the negative feedback control of pancreatic enzyme secretion in conscious rats. Gastroenterology 92: 1987; 449–458.
Gores GJ, LaRusso NF, Miller LJ. Hepatic processing of cholecystokinin peptides, I. Structural specificity and mechanism of hepatic extraction. Am. J. Physiol. 1986; 250: G344-G349.
Mainz DL, Black O, Webster PD. Hormonal control of pancreatic growth. J. Clin. Invest. 1974; 52: 2300–2304.
Morrisset JA, Webster PD. Effect of fasting and feeding on protein synthesis by the rat pancreas. J. Clin. Invest. 1971; 51: 1–8.
Webster PD, Singh Ms Tucker TC, Black O. Effect of fasting and feeding on the pancreas. Gastroenterology 1972; 62: 600–605.
Lee PC, Brooks S, Lebenthal E. Effect of fasting and refeeding on pancreatic enzymes and secretagogue responsiveness in rats. Am. J. Physiol. 1982; 242: G215-G221.
Miyasaka K, Kitani K, Green GM. The sequential changes in pancreatic exocrine function after abdominal surgery in the rat. Pancreas 1986; 1: 347–353.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kurosawa, H., Miyasaka, K. & Kitani, K. Influence of bile flow obstruction vs bile diversion on pancreatic secretion in the conscious rat. Int J Pancreatol 4, 187–197 (1989). https://doi.org/10.1007/BF02931320
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02931320