Folia Microbiologica

, 50:47 | Cite as

Characterization of the alternative sigma factor σG inStreptomyces coelicolor A3(2)

  • B. Ševčíková
  • V. Mazuráková
  • J. Kormanec


Using the previously established two-plasmid system for the identification of promoters recognized by a particular sigma factor, we identified two positive DNA fragments that were active only after inducedsigG, encoding σ factor σG ofStreptomyces coelicolor A3(2). High-resolution S1-nuclease mapping in theEscherichia coli two-plasmid system identified potential promoters, PG45 and PG54, whose sequences were similar to the consensus sequence ofBacillus subtilis promoters recognized by the general stress-response σ factor σB. However, both putative σG-dependent promoters were not active inS. coelicolor. Sequence analysis of the regions potentially governed by the promoters revealed a gene encoding a hypothetical protein SCO5555 and therrnE gene encoding rRNA operon. To confirm thatsigG encodes σ factor, the σG protein was overproduced inE. coli and purified. In anin vitro transcription assay, σG, after complementation withS. coelicolor core RNA polymerase, was able to recognize both σG-dependent promoters and initiate transcription.


Streptomyces rRNA Operon Transcription Assay NdeI Site Transcription Start Point 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  • B. Ševčíková
    • 1
  • V. Mazuráková
    • 1
  • J. Kormanec
    • 1
  1. 1.Institute of Molecular Biology, Center of Excellence for Molecular MedicineSlovak Academy of SciencesBratislavaSlovakia

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