Optimum conditions for the biological production of lactic acid by a newly isolated lactic acid bacterium,Lactobacillus sp. RKY2

  • Young-Jung Wee
  • Jin-Nam Kim
  • Jong-Sun Yun
  • Hwa-Won Ryu


Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose, yeast extract, (NH4)2HPO4, and MnSO4. The optimum pH and temperature for a batch culture ofLactobacillus sp. RKY2 was found to be 6.0 and 36°C, respectively. Under the optimized culture conditions, the maximum lactic acid concentration (153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity (6.21 gL−1h−1) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be above 0.91 g/g. This article provides the optimized conditions for a batch culture ofLactobacillus sp. RKY2, which resulted in highest productivity of lactic acid.


batch fermentation culture conditions homofermentation lactic acid Lactobacillus 


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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2005

Authors and Affiliations

  • Young-Jung Wee
    • 1
  • Jin-Nam Kim
    • 2
  • Jong-Sun Yun
    • 3
  • Hwa-Won Ryu
    • 1
  1. 1.School of Biological Sciences and TechnologyChonnam National UniversityGwangjuKorea
  2. 2.Department of Material Chemical and Biochemical EngineeringChonnam National UniversityGwangjuKorea
  3. 3.BioHelixBiotechnology Industrialization CenterJeonnamKorea

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