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Optimum conditions for the biological production of lactic acid by a newly isolated lactic acid bacterium,Lactobacillus sp. RKY2

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Abstract

Lactic acid is a green chemical that can be used as a raw material for biodegradable polymer. To produce lactic acid through microbial fermentation, we previously screened a novel lactic acid bacterium. In this work, we optimized lactic acid fermentation using a newly isolated and homofermentative lactic acid bacterium. The optimum medium components were found to be glucose, yeast extract, (NH4)2HPO4, and MnSO4. The optimum pH and temperature for a batch culture ofLactobacillus sp. RKY2 was found to be 6.0 and 36°C, respectively. Under the optimized culture conditions, the maximum lactic acid concentration (153.9 g/L) was obtained from 200 g/L of glucose and 15 g/L of yeast extract, and maximum lactic acid productivity (6.21 gL−1h−1) was obtained from 100 g/L of glucose and 20 g/L of yeast extract. In all cases, the lactic acid yields were found to be above 0.91 g/g. This article provides the optimized conditions for a batch culture ofLactobacillus sp. RKY2, which resulted in highest productivity of lactic acid.

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Authors and Affiliations

  1. School of Biological Sciences and Technology, Chonnam National University, 500-757, Gwangju, Korea

    Young-Jung Wee & Hwa-Won Ryu

  2. Department of Material Chemical and Biochemical Engineering, Chonnam National University, 500-757, Gwangju, Korea

    Jin-Nam Kim

  3. BioHelix, Biotechnology Industrialization Center, Naju, 520-811, Jeonnam, Korea

    Jong-Sun Yun

Authors
  1. Young-Jung Wee
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  2. Jin-Nam Kim
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  3. Jong-Sun Yun
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  4. Hwa-Won Ryu
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Correspondence to Hwa-Won Ryu.

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Wee, YJ., Kim, JN., Yun, JS. et al. Optimum conditions for the biological production of lactic acid by a newly isolated lactic acid bacterium,Lactobacillus sp. RKY2. Biotechnol. Bioprocess Eng. 10, 23–28 (2005). https://doi.org/10.1007/BF02931178

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  • DOI: https://doi.org/10.1007/BF02931178

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