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Biotechnology and Bioprocess Engineering

, Volume 12, Issue 3, pp 282–288 | Cite as

Generation of a naïve/synthetic antibody specific to botulinum neurotoxin via motif-grafting

  • Ji-Chul Lee
  • Sun-Young Park
  • Cha-Yong Choi
  • Junho Chung
  • Myung-Shin Lee
Article

Abstract

In this study, we describe a new approach to the production of naïve/synthetic human antibodies against the botulinum neurotoxin (BoNT). First, peptides that bind to BoNT serotype A (BoNT/A) were screened from a phage display of a combinatorial peptide library. One peptide, designated ANT 12-2 (TLPSPLALLTVH), was determined to interact with BoNT/A, as well as with other serotypes of BoNT. This peptide specifically reacted with the native form of BoNT/A, but not with its formalin-inactivated form. Next, a hybrid naïve/synthetic human Fab library was generated via the grafting of a peptide motif from ANT 12-2 into HCDR3 with randomized flanking residues. Through biopanning, the Fab clone, ANTHU-1, which harbors the HCDR3 sequence of VRIQRSPLALLSWGDV, was selected and confirmed in order to retain the same BoNT-binding characteristics as ANT 12-2.

Keywords

botulinum neurotoxin antibody phage display peptide library 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2007

Authors and Affiliations

  1. 1.Interdisciplinary Program of Biochemical Engineering and BiotechnologySeoul National UniversitySeoulKorea
  2. 2.Department of Biochemistry and Molecular Biology and Cancer Research InstituteSeoul National University College of MedicineSeoulKorea
  3. 3.The Armed Forces Medical Research InstituteDaejeonKorea

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