Production and physicochemical characterization of β-glucan produced byPaenibacillus polymyxa JB115
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This study was conducted to develop a bacterial glucan as an animal feed additive. A novel glucan-producing bacterium.Paenibacillus polymyxa JB115, was isolated from Korean soil. The glucan, JB115-BG, produced byP. polymyxa JB115, was confirmed by TLC to be composed of glucose only. By examining FT-IR,1H NMR, and13C NMR spectra, it was proven that JB115-BG has a β-(1→3)- and β-(1→6)-linked glucan structure. The particle size of JB115-BG was distributed in the range of 4–800 μm, with a mean value of 149.1 μm, and its molecular distribution ranged from 6.9∼3,103.7 kDa. It was also observed that 80% of the purified JB115-BG had a molecular distribution above 100 kDa. The obtained results suggest that the glucan JB115-BG can be used as an animal feed additive for the purpose of enhancing immunity.
Keywordsβ-D-glucan exopolysaccharide polysaccharide structure Paenibacillus polymyxa feed additive
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- 1.Ahn, S. G., H. H. Suh, C. H. Lee, S. H. Moon, H. S. Kim, K. H. Ahn, G. S. Kwon, H. M. Oh, and B. D. Yoon (1998) Isolation and characterization of a novel polysaccharide producingBacillus polymyxa A49 KCTC-4648P.J. Microbiol. Biotechnol. 8: 171–177.Google Scholar
- 4.Mansel, P. W. A. (1994) Polysaccharides in skin care,Cosmet. Toilet. 109: 67–72.Google Scholar
- 10.Kawagishi, H., T. Kanao, R. Inagaki, T. Mizuno, K. Shimura, H. Ito, T. Hagiwara, and T. Nakamura (1990) Formolysis of a potent antitumor (1→6) β-d-glucanprotein complex fromAgaricus blazei fruiting bodies and antitumor activity of the resulting products.Carbohydr. Polym. 12: 393–403.CrossRefGoogle Scholar
- 13.Park, J. H., M. S. Kang, H. I. Kim, B. H. Chung, K. H. Lee, and W. K. Moon (2003) Study on immune-stimulating activity of β-glucan isolated from the cell wall of yeast mutantSaccharomyces cerevisiae IS2.Kor. J. Food Sci. Technol. 35: 488–492.Google Scholar
- 14.Seo, H. P., J. M. Kim, H. D. Shin, T. K. Kim, H. J. Chang, B. R. Park, and J. W. Lee (2002) Production of β-1,3/1,6 glucan byAureobasidium pollulans SM-2001.Kor. J. Biotechnol. Bioeng. 17: 376–380.Google Scholar
- 25.Harada, T., M. Masada, K. Fujimori, and I. Maeda (1966) Production of a firm, resilient gel-forming polysaccharide by a mutant ofAlealigenes faecalis var.myxogenes I0C3.Agric. Biol. Chem. 30: 196–198.Google Scholar
- 35.Silvestein, R. M., G. C. Bassler, and T. C. Morrill (1994)Spectrometric Identification of Organic Compounds. 5th ed. Wiley Interscience, NY, USA.Google Scholar
- 36.Altabe, S. G., N. Inon, D. de Mendoza, and R. A. Ugalde (1994) New osmoregulated β(1–3), β(1–6) glucosyltransferase(s) inAzospirillum brasilense.J. Bacteriol. 176: 4890–4898.Google Scholar
- 40.Krakowski, L., J. Krzyzanowski, Z. Wrona, and A. K. Siwicki (1999) The effect of nonspecific immunostimulation of pregnant mares with 1,3/1,6 glucan and levamisole on the immunoglobulins levels in colostrums, selected indices of nonspecific cellular and humoral immunity in foals in neonatal and postnatal period.Vet. Immunol. Immunopathol. 68: 1–11.CrossRefGoogle Scholar