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Phytate degradation by immobilizedSaccharomyces cerevisiae phytase in soybean-curd whey

  • Man-Jin In
  • Kyung-Hee Kim
  • Nam-Soon Oh
Article

Abstract

Saccharomyces cerevisiae CY phytase-producing cells were immobilized in calcium alginate beads and used for the degradation of phylate. The maximum activity and immobilization yield of the immobilized phytase reached 280 mU/g-bead and 43%, respectively. The optimal pH of the immobilized cell phytase was not different from that of the free cells. However, the optimum temperature for the immobilized phytase was 50°C, which was 10°C higher than that of the free cells; pH and thermal stability were enhanced as a consequence of immobilization. Using the immobilized phytase, phytate was degraded in a stirred tank bioreactor. Phytate degradation, both in a buffer solution and in soybean-curd whey mixture, showed very similar trends. At an enzyme dosage of 93.9 mU/g-phytate, half of the phytate was degraded after 1 h of hydrolysis. The operational stability of the immobilized beads was examined with repeated batchwise operations. Based on 50% conversion of the phytate and five times of reuse of the immobilized beads, the specific degradation (g phytate/g dry cell weight) for the immobilized phytase increased 170% compared to that of the free phytase.

Keywords

phytate degradation immobilized phytase Saccharomyces cerevisiae 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2007

Authors and Affiliations

  1. 1.Department of Human Nutrition and Food ScienceChungwoon UniversityHongseongKorea
  2. 2.Department of Food Science and TechnologyKongju National UniversityYesanKorea

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