Abstract
Saccharomyces cerevisiae CY phytase-producing cells were immobilized in calcium alginate beads and used for the degradation of phylate. The maximum activity and immobilization yield of the immobilized phytase reached 280 mU/g-bead and 43%, respectively. The optimal pH of the immobilized cell phytase was not different from that of the free cells. However, the optimum temperature for the immobilized phytase was 50°C, which was 10°C higher than that of the free cells; pH and thermal stability were enhanced as a consequence of immobilization. Using the immobilized phytase, phytate was degraded in a stirred tank bioreactor. Phytate degradation, both in a buffer solution and in soybean-curd whey mixture, showed very similar trends. At an enzyme dosage of 93.9 mU/g-phytate, half of the phytate was degraded after 1 h of hydrolysis. The operational stability of the immobilized beads was examined with repeated batchwise operations. Based on 50% conversion of the phytate and five times of reuse of the immobilized beads, the specific degradation (g phytate/g dry cell weight) for the immobilized phytase increased 170% compared to that of the free phytase.
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References
Reddy, N. R., S. K. Sathe, and D. K. Salunkhe (1982) Phytates in legumes and cereals.Adv. Food Res. 28: 1–92.
Wodzinski, R. J. and A. H. J. Ullah (1996) Phytase.Adv. Appl. Microbiol. 42: 263–302.
Martinez, C., G. Ros, M. J. Periago, G. Lopez, J. Ortuno, and F. Rincon (1996) Phytic acid in human nutrition.Food Sci. Technol. Int. 2: 201–209.
Potter, S. M. (1995) Overview of proposed mechanisms for the hypocholesterolemic effect of soy.J. Nutr. 125: 606S-611S.
Shamsuddin, A. M. (1995) Inositol phosphates have novel anticancer function.J. Nutr. 125: 725S-732S.
Raboy, V. (2001) Seeds for a better future: “low phytate” grains help to overcome malnutrition and reduce pollution.Trends Plant Sci. 6: 458–462.
Pandey, A., G. Szakacs, C. R. Soccol, J. A. Rodriguez-Leon, and V. T. Soccol (2001) Production, purification and properties of microbial phytases.Bioresour. Technol. 77: 203–214.
Ryu, S. and T. G. Park (1998) Thermal stabilization ofAspergillus phytase by L-arginine.Biotechnol. Bioprocess Eng. 3: 32–34.
Gibson, D. M. and A. H. J. Ullah (1988) Purification and characterization of phytase from cotyledons of germinating soybean seeds.Arch. Biochem. Biophys. 260: 503–513.
Simon, O. and F. Igbasan (2002)In vitro properties of phytase from various microbial origins.Int. J. Food Sci. Technol. 37: 813–822.
Mo, A.-Y., S.-M. Park, Y.-S. Kim, M.-S. Yang, and D.-H. Kim (2005) Expression of fungal phytase on the cell surface ofSaccharomyces cerevisiae.Biotechnol. Bioprocess Eng. 10: 576–581.
Seo, S.-W., M.-J. In, and N.-S. Oh (2005) Production and reaction properties of phytase bySaccharomyces cerevisiae CY strain.J. Kor. Soc. Appl. Biol. Chem. 48: 228–232.
Fraser, J. E. and G. F. Bickerstaff (1997) Entrapment in calcium alginate. pp. 61–66. In: G. F. Bickerstaff (ed.).Immobilization of Enzymes and Cells. Humana Press. Totowa, NJ, USA.
Fernandez-Lafuente, R., C. M. Rosell, V. Rodriguez, and J. M. Guisan (1995) Strategies for enzyme stabilization by intramolecular crosslinking with bifunctional reagents.Enzyme Microb. Technol. 17: 517–523.
Quan, C. S., S. D. Fan, and Y. Ohta (2003) Immobilization ofCandida krusei cells producing phytase in alginate gel beads: an application of the preparation ofmyo-inositol phosphates.Appl. Microbiol. Biotechnol. 62: 41–47.
Heinonen, J. K. and R. J. Lahti (1981) A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic phosphatase.Anal. Biochem. 113: 313–317.
Latta, M. and M. Eskin (1980) A simple and rapid colorimetric method for phytate determination.J. Agric. Food Chem. 28: 1313–1315.
Cheetham, P. S. J., K. W. Blunt, and C. Bucke (1979) Physical studies on cell immobilization using calcium alginate gels.Biotechnol. Bioeng. 21: 2155–2168.
Greiner, R. and U. Konietzny (1996) Construction of a bioreactor to produce special breakdown products of phytate.J. Biotechnol. 48: 153–159.
Liu, B. L., C. H. Jong, and Y. M. Tzeng (1999) Effect of immobilization on pH and thermal stability ofAspergillus ficuum phytase.Enzyme Microb. Technol. 25: 517–521.
Ishiguro, T., T. Ono, K. Nakasato, C. Tsukamoto, and S. Shimada (2003) Rapid measurement of phytate in raw soymilk by mid-infrared spectroscopy.Biosci. Biotechnol. Biochem. 67: 752–757.
Lee, D. H., J. M. Kim, H. Y. Shin, S. W. Kang, and S. W. Kim (2006) Biodiesel production using a mixture of immobilizedRhizopus oryzae andCandida rugosa lipases.Biotechnol. Bioprocess Eng. 11: 522–525.
Arruda, L. M. O. and M. Vitolo (1999) Characterization of invertase entrapped into calcium alginate beads.Appl. Biochem. Biotechnol. 81: 23–33.
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In, MJ., Kim, KH. & Oh, NS. Phytate degradation by immobilizedSaccharomyces cerevisiae phytase in soybean-curd whey. Biotechnol. Bioprocess Eng. 12, 348–353 (2007). https://doi.org/10.1007/BF02931055
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DOI: https://doi.org/10.1007/BF02931055