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Biologia Plantarum

, Volume 13, Issue 5–6, pp 405–407 | Cite as

Gametogenesis of tobaccoin vitro

  • J. Bobek
Brief Communication
  • 24 Downloads

Abstract

Excised anthers ofNicotiana tabacum L. were culturedin vitro at the stage of pollen tetrads, which proved in our experiments to be the most suitable initial stage for cultivation, up to the stage of mature pollen grains, using Ito and Stern's nutrient medium, or this medium supplemented with uracil. Germinating capacity of the pollen grains formed and the lengths of pollen tubes were quantitatively evaluated.

Keywords

Pollen Tube Uracil Nutrient Medium Mature Pollen Aniline Blue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Gametogenese u tabákuin vitro

Abstract

Byly pěstovány prašníkyNicotiana tabacum L.in vitro ze stadia pylových tetrád, které se v našich pokusech osvědčilo jako nejspolehlivější výchozí stadium pro kultivaciin vitro, až do stadia zralých pylových zrn, jednak na živné půdě podle Ita a Sterna, jednak na této půdě s uracilem. Kvantitativně byly vyhodnoceny klíčivost vypěstovaných pylových zrn a délka pylových láček.

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References

  1. Ito, M., Stern, H.: Studies of meiosisin vitro. I.In vitro culture of meiotic cells.—Developm. Biol.16: 36–53, 1967.CrossRefGoogle Scholar
  2. Nakata, K., Tanaka, M.: Differentiation of embryoids from developing germ cells in anther culture of tobacco.—Jap. J. Genet.43: 65–71, 1968.CrossRefGoogle Scholar
  3. Sparrow, A. H., Pond, V., Kojan, S.: Microsporogenesis in excised anthers ofTrillium erectum grown on sterile media.—Amer. J. Bot.42: 384–394, 1955.CrossRefGoogle Scholar
  4. Taylor, J. H.: The duration of differentiation in excised anthers.—Amer. J. Bot.37: 137–143, 1950.CrossRefGoogle Scholar
  5. Taylor, J. H.: Incorporation of phosphorus-32 into nucleic acids and proteins during microgametogenesis ofTulbaghia.—Amer. J. Bot.45: 123–131, 1958.CrossRefGoogle Scholar
  6. Taylor, J. H.: Meiosis.—In:Ruhland, W. (ed.): Encyclopedia of Plant Physiology. Vol. 18, Springer-Verlag, Berlin, pp. 344–367, 1967.Google Scholar
  7. Vasil, I. K.: Effect of kinetin and gibberellic acid on excised anthers ofAllium cepa.—Phytomorphology7: 138–149, 1957.Google Scholar
  8. Vasil, I. K.: Nucleic acid and survival of excised anthersin vitro.—Science129: 1487–1488, 1959.CrossRefPubMedGoogle Scholar
  9. Vasil, I. K.: Some new experiments with excised anthers.—In: Plant Tissue and Organ Culture: Symp. int. Soc. Plant Morphologists.—Delhi, pp. 230–238, 1963.Google Scholar
  10. Walker, G. W. R.: The effect of colchicine on microsporogenesis in cultured anthers ofTradescantia paludosa.—Amer. J. Bot.44: 690, 1957.CrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 1971

Authors and Affiliations

  • J. Bobek
    • 1
  1. 1.Institute of Genetics and Plant BreedingResearch Institutes for Plant ProductionPraha-Ruzyně

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