Biologia Plantarum

, Volume 13, Issue 5–6, pp 405–407 | Cite as

Gametogenesis of tobaccoin vitro

  • J. Bobek
Brief Communication


Excised anthers ofNicotiana tabacum L. were culturedin vitro at the stage of pollen tetrads, which proved in our experiments to be the most suitable initial stage for cultivation, up to the stage of mature pollen grains, using Ito and Stern's nutrient medium, or this medium supplemented with uracil. Germinating capacity of the pollen grains formed and the lengths of pollen tubes were quantitatively evaluated.


Pollen Tube Uracil Nutrient Medium Mature Pollen Aniline Blue 
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Gametogenese u tabákuin vitro


Byly pěstovány prašníkyNicotiana tabacum vitro ze stadia pylových tetrád, které se v našich pokusech osvědčilo jako nejspolehlivější výchozí stadium pro kultivaciin vitro, až do stadia zralých pylových zrn, jednak na živné půdě podle Ita a Sterna, jednak na této půdě s uracilem. Kvantitativně byly vyhodnoceny klíčivost vypěstovaných pylových zrn a délka pylových láček.


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Copyright information

© Institute of Experimental Botany 1971

Authors and Affiliations

  • J. Bobek
    • 1
  1. 1.Institute of Genetics and Plant BreedingResearch Institutes for Plant ProductionPraha-Ruzyně

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