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Gametogenesis of tobaccoin vitro

Gametogenese u tabákuin vitro

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Biologia Plantarum

Abstract

Excised anthers ofNicotiana tabacum L. were culturedin vitro at the stage of pollen tetrads, which proved in our experiments to be the most suitable initial stage for cultivation, up to the stage of mature pollen grains, using Ito and Stern's nutrient medium, or this medium supplemented with uracil. Germinating capacity of the pollen grains formed and the lengths of pollen tubes were quantitatively evaluated.

Abstract

Byly pěstovány prašníkyNicotiana tabacum L.in vitro ze stadia pylových tetrád, které se v našich pokusech osvědčilo jako nejspolehlivější výchozí stadium pro kultivaciin vitro, až do stadia zralých pylových zrn, jednak na živné půdě podle Ita a Sterna, jednak na této půdě s uracilem. Kvantitativně byly vyhodnoceny klíčivost vypěstovaných pylových zrn a délka pylových láček.

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References

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Authors and Affiliations

  1. Institute of Genetics and Plant Breeding, Research Institutes for Plant Production, Praha-Ruzyně

    J. Bobek

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  1. J. Bobek
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Address: Praha 6-Ruzyně 507, Czechoslovakia.

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Bobek, J. Gametogenesis of tobaccoin vitro . Biol Plant 13, 405–407 (1971). https://doi.org/10.1007/BF02930932

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  • DOI: https://doi.org/10.1007/BF02930932

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