Advertisement

Biologia Plantarum

, 5:298 | Cite as

Polyphenol oxidase in the roots ofCucurbita pepo L.

  • Miroslav Dvořák
  • Jana Černohorská
Article

Summary

From the roots ofCucurbita pepo L. an acetone preparation was obtained with a polyphenol-oxidase activity towards pyrogallol, less marked towards pyrocatechin and DOPA. It is competitively inhibited by DIECA and KCN, but is not indibited by NaN3, which is evidence that it is a copper enzyme of the polyphenol oxidase type. No quantitative differences between the two variants “NS” and “-Ca” were found nor qualitative differences expressed by the affinity for the substrate.

Keywords

Polyphenol Pyrogallol Polyphenol Oxidase Pyrocatechol Ascorbate Oxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Polyfenoloxydasa v kořenech tykve

Полифенолоксидаза в корнях тыквы

Abstract

Z kořenů tykveCucurbita pepo L., sorta „Kabačky”, byl získán acetonový preparát, který má polyfenoloxydásovou aktivitu vůči pyrogallolu, podstatně menší vůči pyrokatechinu či DOPA. Je kompetitivně inhibován DIECA, dále KCN, není inhibován NaN3, což svědčí pro Cu-enzym polyfenoloxydasového typu. Kvantitativní rozdíly mezi variantami „NS” a „-Ca” nebyly zjištěny, kvalitativní rozdíly, dané afinitou vůči substrátu, nejsou průkazné.

Abstract

Из корней тыквыCucurbita pepo L., сорт «Кабачки» получен ацетоновый препарат, отличающийся полифенолоксидазной активностью по отношению к пирокатехину или кDOPA. Пренарат комнетитивно торможенDIECA, дальше KCN, не торможен NaN3, что говорит в пользу его идентификации как Cu-энзима полифенолоксидазного типа. Количественных различий между вариантами „NS” и «-Ca» не установлено, качественные различия, определяемые сродством по отношению к субстрату недостоверны.

References

  1. Bladegroen, W.: Einführung in die Energetik und Kinetik biologischer Vorgänge. Basel, 1955Google Scholar
  2. Dvořák, M.: The effect of mineral nutrition on the respiratory systems ofCucurbita roots.— Acta Univ. Carol.—Biologica, No. 2, 155–183, 1960.Google Scholar
  3. Dvořák, M.: Participation of ascorbate oxidase in the respiration of theCucurbita roots.—Biol. Plant.5: 287–297, 1963.Google Scholar
  4. James, W. O.: The use of respiratory inhibitors.—Ann. Rev. Plant Physiol.4: 59–90, 1953a.CrossRefGoogle Scholar
  5. James, W. O.: The terminal oxidases of plant respiration.—Biol. Rev.28: 245–260, 1953b.CrossRefGoogle Scholar
  6. James, W. O., Roberts, E. A. H., Beevers, H., De Kock, P. C.: The secondary oxidation of aminoacids by the catecholoxidase of Belladona.—Biochem. J.43: 623–636, 1948.Google Scholar
  7. Keilin, D.: The action of sodium azide on cellular respiration and on some catalytic oxidation reactions.—Proc. R. Soc. (London) B121: 165–173, 1936.CrossRefGoogle Scholar
  8. Warner, C.: A competitive inhibitor of tyrosinase.—Austr. J. Sci. Res. B4: 554–560, 1951.Google Scholar

Copyright information

© Institute of Experimental Botany 1963

Authors and Affiliations

  • Miroslav Dvořák
    • 1
  • Jana Černohorská
    • 1
  1. 1.Department of Plant Physiology and Soil Biology of the Faculty of Natural ScienceCharles UniversityPraha

Personalised recommendations