Advertisement

Evaluation of separation and purification processes in the antibiotic industry

  • Paul R. Bienkowski
  • Douglas D. Lee
  • Charles H. Byers
Session 3-Scientific Note

Abstract

Antibiotic production is a complex capital intensive process, which divides naturally into two segments, fermentation and separation/purification. The separation and purification section is very large as a result of the number of processing steps required (up to 60) and the need to purify and recycle large quantities of organic solvents. Separation and purification is not generic within the antibiotic industry. Not only does each individual antibiotic require a different separation process, but also there are many different separation schemes in use for the same antibiotic. Much research is currently in progress on three relatively new separation techniques on a commercial level, which may lead to substantial reductions in the complexity of the process; chromatography (both conventional preparative HPLC and annular chromatography), supercritical extraction, and various membrane processes.

Index Entries

Antibiotics separation and purification supercritical fluid extraction chromatography 

References

  1. 1.
    Sollmann, T. (1957),A Manual of Pharmacology, 8th ed., W. B. Saunders, London.Google Scholar
  2. 2.
    Perlman, D. (1969),Adv. in Appl. Microb. 11, 159, Academic, New York.Google Scholar
  3. 3.
    Stinson, S. C. (1985),C & E News,16, 25.Google Scholar
  4. 4.
    Kertes, A. S. (1985),Solubility Data Series,16 & 17, Pergamon, NY.Google Scholar
  5. 5.
    Atkinson, B., and Mavituna, F. (1983),Biochem. Eng. and Biotechnol. Handbook, Nature Press, London.Google Scholar
  6. 6.
    {au{fnLepetit}, {gnS. P. A.}}Production of the Antibiotic Tetracycline by Fermentation, British Patent #775 139, May 22, 1957.Google Scholar
  7. 7.
    Minieri, P. P., and Sokol, H.Process for the Preparation of Tetracycline and Chlortetracycline, United States Patent # 2,734,018 February 7, 1956.Google Scholar
  8. 8.
    McGhee, W. J., and Megna, J. C.Process for the Production of Tetracycline, United States Patent #2,776,243 January 1, 1957.Google Scholar
  9. 9.
    Okabe, M., and Aiba, S. (1975),J. Ferment. Technol. 53(10), 730.Google Scholar
  10. 10.
    Elder, A. L., ed. (1970), AlChE Symp. Ser., 66, 1.Google Scholar
  11. 11.
    Hugo, W. B., and Russel, A. D. (1977),Pharm. Microb., Blackwell, London.Google Scholar
  12. 12.
    Inman, F. N. (1984),Filtr. Sep.,May/June, 165.Google Scholar
  13. 13.
    Peppier, H. J., and Perlman, D. (1979),Microb. Technol., 2nd ed., Academic, NY.Google Scholar
  14. 14.
    Niss, H. F.Process for the Manufacture of Cephalosporin C, United States Patent #3,539,694 June 23, 1967.Google Scholar
  15. 15.
    Wildfeuer, M. E. (1985),Pur. Ferment. Prod., 155.Google Scholar
  16. 16.
    Hockenhold, D. J. D. (1959),Fermentation of the Tetracyclines, Interscience, NY.Google Scholar
  17. 17.
    Plues, S.Antibiotic Purification Process, United States Patent #3,983,108 September 28, 1976.Google Scholar
  18. 18.
    Gavrilescu, M., Pal, C, User, S., Tonescu, S., and Margineanu, N.Process for the Isolation and Purification of Tetracycline, British Patent #1 368 668 October 2, 1974.Google Scholar
  19. 19.
    Kalyanpur, M., Seka, W., and Siwak, M. (1984),SA Filtr.,Nov., 8.Google Scholar
  20. 20.
    Cantwell, A. M., Calderone, R., and Sienko, M. (1984),J. Chrom.,316, 133.CrossRefGoogle Scholar
  21. 21.
    Dwyer, J., Findeisen, C., and Seka, W. (1984),Pharm. Tech.,8(9), 100.Google Scholar
  22. 22.
    Dwyer, J. (1984),World Biotech. Rept., 651.Google Scholar
  23. 23.
    Knox, J. H., and Jurand, J. (1975),J. Chrom. 110, 103.CrossRefGoogle Scholar
  24. 24.
    Yoshikawa, T. T., Maltra, S. K., Schotz, M. C., and Guze, L. B. (1980),Rev. Infec. Dis. 2(2), 169.Google Scholar
  25. 25.
    Watson, I. D., Platt, D. J., McIntosh, S. J., Stewart, M. J., and Cohen, H. N. (1982),Clinical Biol. Dev. HPLC, 180.Google Scholar
  26. 26.
    White, E. R., and Zarembo, J. E. (1981),J. Antib.,24(7), 836.Google Scholar
  27. 27.
    Kalyanpur, M., Seka, W., and Siwak, N. (1985),Dev. Ind. Micro. 26, 455.Google Scholar
  28. 28.
    Miller, R. D., and Neuss, N. (1976),J. Antib. 29(9), 902.Google Scholar
  29. 29.
    Mascone, C. F. (1987),Chem. Eng. 19, 21.Google Scholar
  30. 30.
    Stewart, G. T.Purified Cephalosporins and Their Production, British Patent #1232 656 May 23, 1968.Google Scholar
  31. 31.
    Blackburn, D. W.Cephalosporin Purification Process, United States Patent # 4,028,755 June 7, 1977.Google Scholar
  32. 32.
    Scott, C. D., Spence, R. D., and Sission, W. G. (1976),J. Chromatogr. 126, 381.CrossRefGoogle Scholar
  33. 33.
    Begovich, J. M., and Sisson, W. G. (1984),AlChE J. 30, 705.Google Scholar
  34. 34.
    Larson, K. A., and King, M. L. (1986),Biotec. Prog.,2(2), 73.CrossRefGoogle Scholar
  35. 35.
    Cohen, H.Isolation and Purification of Antibiotics, German Patent #33 18 194 May 19, 1983.Google Scholar

Copyright information

© Humana Press Inc. 1988

Authors and Affiliations

  • Paul R. Bienkowski
    • 1
  • Douglas D. Lee
    • 2
  • Charles H. Byers
    • 2
  1. 1.Department of Chemical EngineeringUniversity of TennesseeUSA
  2. 2.Chemical Technology DivisionOak Ridge National LaboratoryUSA

Personalised recommendations