Abstract
A new type of reactor, the attrition bioreactor, has been developed to increase the rate of the enzymatic hydrolysis of cellulose and also to cut pretreatment costs. It was found that the attrition bioreactor could be operated continuously or semicontinuously in conjunction with a membrane filter to produce a high cellulose conversion rate and low enzyme consumption. The membrane filter served to contain the enzyme and cellulose within the reactor while allowing sugar to permeate as a product.
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Abbreviations
- B :
-
The flow rate of the bleeding stream, L/h.
- CE :
-
Enzyme concentration, g/L.
- CP :
-
Product sugar concentration, g/L.
- Cs :
-
Substrate concentration, g/L.
- F :
-
Medium flow rate, L/h.
- L :
-
The flow rate of the filtrate stream, L/h.
- rP :
-
The rate of product formation by enzymatic hydrolysis, g/L h.
- rs :
-
The rate of substrate consumption by enzymatic hydrolysis, g/L h.
- t :
-
Time, h.
- V :
-
The volume of the medium in the reactor, L.
- Yp/s :
-
The yield constant, g of product formed/g of substrate consumed.
- 0:
-
Initial value.
- B:
-
Bleeding stream.
- F:
-
Feed stream.
- L:
-
Filtrate stream.
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Lee, J.M., Wolf, J.H. Continuous attrition bioreactor with enzyme recycling for the bioconversion of cellulose. Appl Biochem Biotechnol 18, 203–215 (1988). https://doi.org/10.1007/BF02930826
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DOI: https://doi.org/10.1007/BF02930826