Summary
Coliform cells resting in phosphate buffer at 30° C show protein turnover at approximately 2% per hour.
In this turnover reaction the incorporation of amino acids into protein is inhibited by sodium azide and chloramphenicol, but the release of amino acids from protein is not inhibited by either chloramphenicol or sodium azide.
In resting cells both ribosomes and soluble proteins lose amino acid residues, but amino acids are only incorporated into the soluble proteins.
From resting cells which are releasing amino acid residues from protein to the cold trichloroacetic acid-soluble fraction peptides have been isolated. These peptides contain amino acids at the same specific radioactivity as those in the cell protein being degraded. The peptides are probably intermediates in protein breakdownin vivo.
The resting bacteria contain proteolytic activities, these appear to be associated with the ribosomes and become active only when the ribosome is disrupted.
This study was made during the tenure of an Overseas Science Research Scholarship of the Exhibition of 1851, and was also supported by a grant from the Medical Research Council to defray the costs of isotopes. Dr. Kenneth McQuillen gave stimulating encouragement throughout the study and Professor E. F. Gale afforded the generous hospitality of his laboratory.
Abstract
Инкдбируeмыe в фосфатном буфeрe клeтки колиобразнопо орпанизма обнаруживают «turnover» при скорости приблизитeлЬно 2%/чаА. В ходe этопо прeвращeния вклозeниe аминокислот в бeлки угнeтаeтся азидом натрия и влорамфeниколом. Однако на освобождeниe аминокислот из бeлков ни хлорамфeникол, ни азид натрия нe дeйствуют. В клeтках в стадии покоя аминокислоты освобождаются из рибосом и растворимы e бeлков, но вклочаются только в растворимыe бeлки. Из клeток в стадии покоя, выдeляющих аминокиАлоты бeлков во фракцию, eаствориму044E; в eолодной тритлорукАусной кислотe, были выдeлeны пeптиды, содeржайиe аминокислотK А такой жe спeцифичeской радиоактивноАтью, как аминокислоты в дeградирующих клeточных бeлоах. По-видимому, ати пeптиды являются промeжуточным продукРос дeгЄадации бeлков in vivo. В стадии покоя бактeeии обладают протeолитичeскойак тивностью, которая, по-видимому, илeeт оРношeниe к рибосомам и проявлпeРся Родько посдe их разрушeния.
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Proctor, M.H. Protein turnover in resting bacteria. Folia Microbiol 7, 207–215 (1962). https://doi.org/10.1007/BF02930765
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DOI: https://doi.org/10.1007/BF02930765