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Purification and partial characterization of two lectin isoforms fromCratylia mollis mart. (camaratu bean)

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Abstract

Two additional electrophoretically distinct molecular forms, isoforms (iso) 2 and 3, with lectin properties were isolated fromCratylia mollis Mart, seeds (FABACEAE), by extraction with 0.15M NaCl and ammonium sulfate fractionation, followed by chromatography on Sephadex G-75 and Bio-Gel P-200 (iso 2), as well as CM-Cellulose and Sephadex G-75 (iso 3). Both isoforms were human group nonspecific and showed distinct specificity. Polyacrylamide gel electrophoresis resolved iso 2 and 3 in polypeptides of apparent mol wts 60 and 31 kDa, respectively; a distinct isoelectric focusing pattern was obtained for iso 2 and 3, under denaturing and reducing conditions.

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Authors and Affiliations

  1. Departamento de Bioquunica, Centro de Ciências Biológicas,Uniuersidade Federal de Pernambuco (UFPE), Au. Moraes Rego,s/n, Cidade Unioersitária, 50730, Recife,PE, Brazil

    Patricia M. G. Paiva & Luana C. B. B. Coelho

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  1. Patricia M. G. Paiva
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  2. Luana C. B. B. Coelho
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Paiva, P.M.G., Coelho, L.C.B.B. Purification and partial characterization of two lectin isoforms fromCratylia mollis mart. (camaratu bean). Appl Biochem Biotechnol 36, 113–118 (1992). https://doi.org/10.1007/BF02929691

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  • DOI: https://doi.org/10.1007/BF02929691

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