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Folia Microbiologica

, Volume 4, Issue 5, pp 312–315 | Cite as

The effect of DL-β-methylaspartic acid on the growth ofEscherichia coli

  • K. Vereš
  • J. Chaloupka
  • M. Dobiásová
  • L. Kocmanová
Folia Microbilogica

Summary

DL-β-methylaspartic acid was prepared and its two isomers separated. The more soluble erythro-form stimulated the growth ofEscherichia coli in a synthetic medium at a concentration of 1–5 μmoles/ml. The less soluble fraction containing the threo-form inhibited the growth ofEscherichia coli at the given concentrations. The growth inhibition was evidenced by a prolongation of the lag phase; the growth rate in the logarithmic phase and the maximum number of cells were not affected by the analogue. The inhibitory effect caused by the threo-form of β-methylaspartic acid could be suppressed by the addition of aspartic acid.

Keywords

Glutamic Acid Aspartic Acid Amphenicol Analogue Concentration Aspartie Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Эффект дл л-methylaspartic кислоты на рост (itescherichia титр)

Abstract

ВЫла кислота была под готовлена и двух его и зомеры отделить друг от друга. Больше erythro-раст воримой форме стимул ировали рост (itEscherichia титр) в синтетических средн ие на концентрацию 1–5 gmmoles мл. Менее растворимы е фракции, содержащие threo-форма препятствует росту из (itEscherichia коли) на дан ной концентрации. Инг ибирование роста сви детельствует путем п ролонгации лаг фазы; Темпы роста в логари фмической фазы и Макс имальное количество ячеек не были пострад авшим от аналога. Торм озящий последствия, в ызванные threo-форма methylaspartic ки слоты могут быть пода влены Помимо этого из аспарагиновой кисло ты.

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References

  1. Barker, H. A., Smyth, R. D., Wilson, R. M.:Bole of β-methylaspartate in metabolism of glutamate by Clostridium tetanomorphum. Fed. Proc. 17: 186, 1958a.Google Scholar
  2. Barker, H. A., Smyth, R. D., Wawskiewicz, E. J., Lee, M. N., Wilson, R. M.:Enzymic preparation and characterization of an α-L-β-methylaspartic acid. Arch. Biochem. Biophys. 78: 468, 1958b.PubMedCrossRefGoogle Scholar
  3. Dakin, H. D.:On methylaspartic acids and their methylation. J. biol. Chem. 141: 945, 1941.Google Scholar
  4. Mandelstam, J.:The free aminoacids in growing and non-growing populations of Escherichia coli. Biochem. J. 69: 103, 1958.PubMedGoogle Scholar
  5. Martin, G. J.:Biological antagonism. The Blakiston New York, 1951.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1959

Authors and Affiliations

  • K. Vereš
    • 1
  • J. Chaloupka
    • 1
  • M. Dobiásová
    • 1
  • L. Kocmanová
    • 1
  1. 1.Radioisotope Laboratory and Department of Microbiology, Institute of BiologyCzechoslovak Academy of SciencesPraha

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