Folia Microbiologica

, 30:433 | Cite as

Morphological and colour mutants ofTrichoderma viride: Characterization and complementation

  • A. Fargašová
  • M. Sipicpzki
  • V. Betina


A total of 26 morphological and colour mutants ofTrichoderma viride were characterized. They were divided into three groups based on morphology, pigmentation, growth rates and intensity of conidiation. Complementation analysis of colour mutants and mutants with disturbances in conidiation showed that after anastomosis and protoplast fusion only heterokaryotes are formed while no nuclear migration and diploidization takes place.


Parent Strain Agar Block Protoplast Fusion Nuclear Migration Mutant Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Arpai J., Stuchlík V.: Use of isolator for yeast selection. (In Slovak)Kvasný průmysl 3, 182 (1957).Google Scholar
  2. Betina V.: Photoinduced conidiation inTrichoderma viride. Internat.J.Microbiol. 2, 55 (1984).Google Scholar
  3. Betina V., Spišiaková J.: Suppression of photoinduced sporulation inTrichoderma viride by inhibitors.Folia Microbiol. 21, 362 (1976).CrossRefGoogle Scholar
  4. Betina V., Zajacová J.: Regulation of periodicity and intensity of photoinduced conidiation ofTrichoderma viride.Folia Microbiol. 23, 453 (1978a).CrossRefGoogle Scholar
  5. Betina V., Zajacová J.: Inhibition of photo-inducedTrichoderma viride conidiation by inhibitors of RNA synthesis.Folia Microbiol. 23, 460 (1978b).CrossRefGoogle Scholar
  6. Bbtina V., Spišiaková J., Zajacová J.: Photoinduced conidiation inTrichoderma viride is prevented by inhibitors of RNA and protein synthesis.12th FEBS Meeting, Dresden 1978.Google Scholar
  7. Betina V., Sedmera P., Vokoun J., Podojil M.: Anthraquinone pigments from a conidiation mutant ofTrichoderma viride. Experientia (1985).Google Scholar
  8. Bojnaňská A., Betina V., Nemec P.: Selection of color mutants ofTrichoderma viride Pers. ex Fries.Proc. Gryp. Symp. SAS 171 (1978).Google Scholar
  9. Bojňanská A., Sipiczki M., Ferenczy L.: Characterization of conidiation mutants inTrichoderma viride by hyphal anastomosis and protoplast fusion.Acta Microbiol.Hung. 27, 305 (1980).Google Scholar
  10. Clutterbuck A.J.: A mutational analysis of conidial development inAspergillus nidulans.Genetics 63, 317 (1969).PubMedGoogle Scholar
  11. Fargašová A., Betina V.: Acquisition of small colonies ofTrichoderma viride for genetic analysis.Folia Microbiol. 30, 458 (1985).Google Scholar
  12. Galun E., Gressel J.: Morphogenesis inTrichoderma: Suppresion of photoinduction by 5-fluorouracil.Science 151, 696 (1966).PubMedCrossRefGoogle Scholar
  13. Greenshpan D., Galun E.: Morphogenesis inTrichoderma: Autonomous and nonautonomous pigmentation in heterokaryons of color mutants inTrichoderma viride.Amer. J.Bot. 58, 287 (1971).CrossRefGoogle Scholar
  14. Gressel J., Galun E.: Effect of 5-fluorouracil on soluble RNA ofTrichoderma.Biochem.Biophys. Res.Commun. 24, 162 (1966).PubMedGoogle Scholar
  15. Gressel J., Galun E.: Morphogenesis inTrichoderma: Photoinduction and RNA.Develop.Biol. 15, 575 (1967).PubMedCrossRefGoogle Scholar
  16. Gressel J., Hartmann K.M.: Morphogenesis inTrichoderma: Action spectrum of photoinduced sporulation.Planta 79, 271 (1968).CrossRefGoogle Scholar
  17. Horwitz B.A., Gressel J.: Elevated riboflavin requirement for postphotoinductive events in sporulation ofTrichoderma auxotroph.Plant.Physiol. 71, 200 (1983).PubMedCrossRefGoogle Scholar
  18. Horwitz B.A., Gressel J., Malkin S.: The quest forTrichoderma cryptochrome, in H. Senger:The Blue Light Syndrome II. Springer, Berlin 1985.Google Scholar
  19. Martinelli L.D., Clutterbuck A.J.: A quantitative survey of conidiation mutants inAspergillus nidulans.J.Gen.Microbiol. 69, 261 (1971).PubMedGoogle Scholar
  20. Oliver P.T.P.: Conidiophore and spore development inAspergillus nidulans.J.Gen.Microbiol. 73, 45 (1972).PubMedGoogle Scholar
  21. Picataggio S.K., Achamhart D.H.J., Montenecourt B.S., Eveleigh D.E.: Sphaeroplast formation and regeneration inTrichoderma reesei.Eur.J.Appl.Microbiol.Biotechnol. 17, 121, (1983).CrossRefGoogle Scholar
  22. Sychev P.A., Khizhenkov P.K.: Absorption spectra ofTrichoderma (Pers. ex Fries) pigments.Microbiol.Zh. 40, 367 (1978).Google Scholar
  23. Trevithick J.R., Metzenberg R.L: Genetic alteration of pore size and other properties of theNeurospora cell wall.J.Bacteriol. 92, 1106 (1966a).Google Scholar
  24. Trevithick J.R., Metzenberg R.L.: Molecular sieving byNeurospora cell wall during secretion of invertase isozymes.J.Bacteriol. 92, 1010 (1966b).PubMedGoogle Scholar
  25. Trinci A.P.J.: A kinetic study of the growth ofAspergillus nidulans and other fungi.J.Gen.Microbiol. 57, 11 (1969).PubMedGoogle Scholar
  26. Weinman-Greenshpan D., Galun E.: Complementation in nonconidiating mutants ofTrichoderma.J.Bacteriol. 99, 802 (1969).PubMedGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1985

Authors and Affiliations

  • A. Fargašová
    • 1
  • M. Sipicpzki
    • 2
  • V. Betina
    • 1
  1. 1.Department of Environmental Chemistry and TechnologySlovak Polytechnical UniversityBratislavaCzechoslovakia
  2. 2.Deparment of MicrobiologyAttila József UniversitySzegedHungary

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