Abstract
Isoleucine added to the cultivation medium ofStreptomyces cinnamonensis C-10Q-5 induced a relative increase of the production of monensin B at the expense of monensin A.U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of l-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(i) of propionate. In regulatory mutants insensitive to 2-amino-3-cblorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2—12 % in the presence of isoleucine which was associated with a decrease of monensin A content by 14—52 %. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.
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The authors wish to thank Dr. M. Havránek (Institute of Nuclear Biology and Radiochemistry,Czech.Acad.Sci., Prague) for the synthesis of sodium 1-13C-2-methylbutyrate.
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Pospíšil, S., Sedmera, P., Krumphanzl, V. et al. Biosynthesis of monensins a and b: the role of isoleucine. Folia Microbiol 31, 8–14 (1986). https://doi.org/10.1007/BF02928674
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DOI: https://doi.org/10.1007/BF02928674