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Biosynthesis of monensins a and b: the role of isoleucine


Isoleucine added to the cultivation medium ofStreptomyces cinnamonensis C-10Q-5 induced a relative increase of the production of monensin B at the expense of monensin A.U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of l-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(i) of propionate. In regulatory mutants insensitive to 2-amino-3-cblorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2—12 % in the presence of isoleucine which was associated with a decrease of monensin A content by 14—52 %. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.

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Correspondence to S. Pospíšil or P. Sedmera or V. Krumphanzl or Z. Vaněk.

Additional information

The authors wish to thank Dr. M. Havránek (Institute of Nuclear Biology and Radiochemistry,Czech.Acad.Sci., Prague) for the synthesis of sodium 1-13C-2-methylbutyrate.

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Pospíšil, S., Sedmera, P., Krumphanzl, V. et al. Biosynthesis of monensins a and b: the role of isoleucine. Folia Microbiol 31, 8–14 (1986).

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  • Butyrate
  • Isoleucine
  • Monensin
  • Amino Acid Analogue
  • Norleucine