Folia Microbiologica

, Volume 31, Issue 1, pp 8–14 | Cite as

Biosynthesis of monensins a and b: the role of isoleucine

  • S. Pospíšil
  • P. Sedmera
  • V. Krumphanzl
  • Z. Vaněk


Isoleucine added to the cultivation medium ofStreptomyces cinnamonensis C-10Q-5 induced a relative increase of the production of monensin B at the expense of monensin A.U-14C-Isoleucine was found not to be a specific monensin B precursor. The incorporation of l-13C-2-methylbutyrate into monensins A and B showed the label to be evenly incorporated in both products at carbon atoms originating from C(i) of propionate. In regulatory mutants insensitive to 2-amino-3-cblorobutyrate isoleucine influenced the production of monensins only slightly but strains resistant to 2-aminobutyrate and norleucine decreased their total production by 2—12 % in the presence of isoleucine which was associated with a decrease of monensin A content by 14—52 %. The inhibitory effect of isoleucine on the biosynthesis of valine, a specific precursor of the butyrate unit of monensin A, is discussed.


Butyrate Isoleucine Monensin Amino Acid Analogue Norleucine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1986

Authors and Affiliations

  • S. Pospíšil
    • 1
  • P. Sedmera
    • 1
  • V. Krumphanzl
    • 1
  • Z. Vaněk
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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