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Physiological and biochemical aspects of tolerance of three grass species to varying Na+/Ca2+ ratios

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Biologia Plantarum

Abstract

The effects of decreasing Ca2+ concentrations (Na/Ca ratios were 24, 49, 99 and 199) of the saline growth medium (NaCl concentration 200 mM) on three grass speciesCenchrus pennisetiformis Hochst. & Steud,Leptochloa fusca L. Kunth. andPcmicum turgidum Forssk. were assessed after 7 weeks growth in sand culture.L. fusca produced the highest dry biomass of all the species at varying Na/Ca ratios. Number of tillers per plant and number of green leaves per tiller were reduced significantly only inC. pennisetiformis. Leaf water potential ofC. pennisetiformis decreased at all external Na/Ca ratios, whereas inL. fusca it decreased only at an Na/Ca ratio of 99. Leaf osmotic potential ofL. fusca consistently decreased at all Na/Ca ratios, whereas that of the other two species remained unchanged. The shoot and root total sugars of all species remained unaffected at all decreasing Ca2+ concentrations. InP. turgidum chlorophyllb and total chlorophyll decreased consistently at all Na/Ca ratios, but inL. fusca they decreased only at the highest Na/Ca ratios. The leaf soluble proteins of all the species remained unaffected at all Na/Ca ratios. The leaf free amino acids decreased significantly inL. fusca with the increase in Na/Ca ratios. The leaf proline content was only decreased inL. fusca at the highest Na/Ca ratio. The significant correlations between the growth of the three grass species and other variables determined in this study were not found.

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References

  • Ashraf, M.: The effect of NaCl on water relations, chlorophyll, and protein and proline contents of two cultivars of black gram (Vigna mungo L.). - Plant Soil119: 205–210, 1989.

    Article  CAS  Google Scholar 

  • Ashraf, M. Bokhari, M.H.: Biological approach for economic utilization of the Cholistan desert. - Biologia33(2): 27–34, 1987.

    Google Scholar 

  • Ashwell, G.: Colorimetric analysis of sugars. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 3. Pp. 84–85. Academic Press, New York 1957.

    Google Scholar 

  • Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. -Plant Soil39: 205–207, 1973.

    Article  CAS  Google Scholar 

  • Epstein, E.: The essential role of calcium in selective ion transport by plant cells. - Plant Physiol.36: 437–444, 1961.

    PubMed  CAS  Google Scholar 

  • Flowers, T.J, Troke, P.F., Yeo, A.R.: The mechanism of salt tolerance in halophytes. - Annu. Rev. Plant Physiol.28: 89–121, 1977.

    Article  CAS  Google Scholar 

  • Greenway, H., Munns, R.: Mechanism of salt tolerance in non-halophytes. - Annu. Rev. Plant Physiol.31: 149–190, 1980.

    Article  CAS  Google Scholar 

  • Hamilton, P.B., Van Slyke, D.D.: Amino acid determination with ninhydrin. - J. biol. Chem.150: 231–233, 1943.

    CAS  Google Scholar 

  • Kawasaki, T., Moritsugu, M.: Effect of calcium on salt injury in plants. II. Barley and rice. Ber. Ohara Inst. landwirtschaft. Biol., Okayama Univ.17: 73–81, 1978.

    CAS  Google Scholar 

  • Kent, L.M., Läuchli, A.: Germination and seedling growth of cotton: Salinity-calcium interactions. - Plant Cell Environ.8: 155–159, 1985.

    Article  CAS  Google Scholar 

  • Leopold, A.C., Willing, R.D.: Evidence for toxicity effects of salt on membranes. - In: Staples, R.C., Toenniessen, G.H. (ed.): Salinity Tolerance in Plants: Strategies for Crop Improvement. Pp. 67–91. John Wiley, New York 1984.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.H. Farr, A.L., Randel, R.J.: Protein measurement with Folin phenol reagent.-J. biol. Chem.193: 265–275, 1951.

    PubMed  CAS  Google Scholar 

  • Maas, E.V., Grieve, C.M.: Sodium-induced calcium deficiency in salt stressed corn. - Plant Cell Environ.10: 559–564, 1987.

    Google Scholar 

  • Malik, K.A., Aslam, Z., Naqvi, M.: Kallar Grass - A Plant for Saline Land. - The Nuclear Institute for Agriculture and Biology, Faisalabad 1986.

    Google Scholar 

  • Moftah, A.E., Michel, B.E.: The effect of sodium chloride on solute potential and prolinc accumulation in soybean leaves.- Plant Physiol.83: 238–240, 1987.

    Article  PubMed  CAS  Google Scholar 

  • Rains, D.W.: Salt transport by plants in relation to salinity. - Annu. Rev. Plant. Physiol.23: 267–388, 1972.

    Article  Google Scholar 

  • Rains, D.W.: Salt tolerence - New development. - In: Manassah, J.T., Briskey, E.J. (ed.): Advances in Food Producing Systems for Arid and Semi Arid Lands. Pp. 431–456. Academic Press, New York 1981.

    Google Scholar 

  • Snedecor, G.W., Cochran. W.G.: Statistical Methods. Seventh Edition. The Iowa State, University Press, Ames, Iowa 1980.

    Google Scholar 

  • Witham, F.H., Blaydes, D.F., Devlin, R.M.: Experiments in Plant Physiology. - Van Norstand Reinhold Co., New York 1971.

    Google Scholar 

  • Wyn-Jones R.G.: Salt tolerance. - In: Johnson, C.B. (ed.): Physiological Processes Limiting Plant Productivity. Pp. 271–292. Butterworths, London 1980.

    Google Scholar 

  • Yeo, A.R., Flowers, T.J.: The absence of an effect of the Na/Ca ratio on sodium chloride uptake by rice (Oryza saliva L). - New Phytol.99: 81–90, 1985.

    Article  CAS  Google Scholar 

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Ashraf, M., Naqvi, M.I. & Zafar, Z.U. Physiological and biochemical aspects of tolerance of three grass species to varying Na+/Ca2+ ratios. Biol Plant 35, 425–433 (1993). https://doi.org/10.1007/BF02928521

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