Biologia Plantarum

, 35:425 | Cite as

Physiological and biochemical aspects of tolerance of three grass species to varying Na+/Ca2+ ratios

  • M. Ashraf
  • M. I. Naqvi
  • Z. U. Zafar


The effects of decreasing Ca2+ concentrations (Na/Ca ratios were 24, 49, 99 and 199) of the saline growth medium (NaCl concentration 200 mM) on three grass speciesCenchrus pennisetiformis Hochst. & Steud,Leptochloa fusca L. Kunth. andPcmicum turgidum Forssk. were assessed after 7 weeks growth in sand culture.L. fusca produced the highest dry biomass of all the species at varying Na/Ca ratios. Number of tillers per plant and number of green leaves per tiller were reduced significantly only inC. pennisetiformis. Leaf water potential ofC. pennisetiformis decreased at all external Na/Ca ratios, whereas inL. fusca it decreased only at an Na/Ca ratio of 99. Leaf osmotic potential ofL. fusca consistently decreased at all Na/Ca ratios, whereas that of the other two species remained unchanged. The shoot and root total sugars of all species remained unaffected at all decreasing Ca2+ concentrations. InP. turgidum chlorophyllb and total chlorophyll decreased consistently at all Na/Ca ratios, but inL. fusca they decreased only at the highest Na/Ca ratios. The leaf soluble proteins of all the species remained unaffected at all Na/Ca ratios. The leaf free amino acids decreased significantly inL. fusca with the increase in Na/Ca ratios. The leaf proline content was only decreased inL. fusca at the highest Na/Ca ratio. The significant correlations between the growth of the three grass species and other variables determined in this study were not found.


Grass Species Proline Content Total Chlorophyll Leaf Water Potential Fusca 
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Copyright information

© Institute of Experimental Botany 1993

Authors and Affiliations

  • M. Ashraf
    • 1
  • M. I. Naqvi
    • 1
  • Z. U. Zafar
    • 1
  1. 1.Institute of Pure and Applied BiologyBahauddin Zakariya UniversityMultanPakistan

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