Effects of seasons and hormones on pre- and postfertilization barriers of crossability andin vitro hybrid development betweenVigna unguiculata andV. mungo crosses
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Effect of two different seasons and hormones on pre- and postfertilization barriers in crossability were studied inVigna mungo, V. unguiculata and their crosses. Significant differences between the two seasons for pollen fall, pollen germination, tube growth and pod set were observed. In kharif season with an average temperature of 26 to 30 °C and 75 to 85 % humidity all the characters showed higher values than in the spring season with a higher temperature of 32 to 36 °C and low humidity of 40 to 50 %. Application of gibberellic acid (GA) alone and a combination of gibberellic acid, naphthaleneacetic acid (NAA) and kinetin (KIN) significantly increased the pollen fall by 7–13 %, pollen germination by 10–12 % and pollen tube growth by 27–30 %. More pollen tube abnormalities in interspecific crosses only indicates partly incompatible reaction of pollen tube in the stylar tisue. Hormonal treatments, especially GA, significantly increased the pod set and pod harvest by 20 % and 34 %, respectively. Higher pod harvest was seen in crosses withV. unguiculata as female parent. GA treatment enhanced pollen germination and pollen tube growth, and by partially overcoming embryo abortion for 10 to 12 d, immature embryos were successfully rescued forin vitro production of hybrid plantlets.
KeywordsPollen Tube Gibberellic Acid Pollen Tube Growth Pollen Germination Spring Season
benzylaminopurine, 2,4-D - 2,4 dichlorophenoxyacetic acid
medium of Murashige and Skoog (1962)
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