Effects of seasons and hormones on pre- and postfertilization barriers of crossability andin vitro hybrid development betweenVigna unguiculata andV. mungo crosses
Effect of two different seasons and hormones on pre- and postfertilization barriers in crossability were studied inVigna mungo, V. unguiculata and their crosses. Significant differences between the two seasons for pollen fall, pollen germination, tube growth and pod set were observed. In kharif season with an average temperature of 26 to 30 °C and 75 to 85 % humidity all the characters showed higher values than in the spring season with a higher temperature of 32 to 36 °C and low humidity of 40 to 50 %. Application of gibberellic acid (GA) alone and a combination of gibberellic acid, naphthaleneacetic acid (NAA) and kinetin (KIN) significantly increased the pollen fall by 7–13 %, pollen germination by 10–12 % and pollen tube growth by 27–30 %. More pollen tube abnormalities in interspecific crosses only indicates partly incompatible reaction of pollen tube in the stylar tisue. Hormonal treatments, especially GA, significantly increased the pod set and pod harvest by 20 % and 34 %, respectively. Higher pod harvest was seen in crosses withV. unguiculata as female parent. GA treatment enhanced pollen germination and pollen tube growth, and by partially overcoming embryo abortion for 10 to 12 d, immature embryos were successfully rescued forin vitro production of hybrid plantlets.
KeywordsPollen Tube Gibberellic Acid Pollen Tube Growth Pollen Germination Spring Season
benzylaminopurine, 2,4-D - 2,4 dichlorophenoxyacetic acid
medium of Murashige and Skoog (1962)
Unable to display preview. Download preview PDF.
- Al-Yasiri, S.A., Coyne, D.P.: Effect of growth regulators in delaying pod abortion in the interspecific crossPhaseolus vulgaris×P. acutifolius. - Crop Sci.4: 433–435, 1964.Google Scholar
- Baker, L.R., Chen, N.C., Park, H.G.: Effect of an immune suppressant on an interspecific cross of the genusVigna. - Hort. Sci.10: 313, 1975.Google Scholar
- Chowdhury, R.K., Chowdhury, J.B.: Intergeneric hybridization betweenVigna mungo (L) Hepper andPhaseolus calcaratus Roxb. - Indian J. agr. Sci.47: 117–121, 1977.Google Scholar
- Dumas, C., Gaude, T.: Stigma- pollen recognition: a new look. - Acta Soc. Bot. Pol.50(Symp. Adv. Plant Cytoembryology): 235–247, 1981.Google Scholar
- Gupta, M., Murty, Y.S.:In vitro hormonal regulation of pollen germination and pollen tube growth inVicia faba L.- Biovigyanam11: 156–158, 1985.Google Scholar
- Hallerlein, A.J., Clayberg, CD., Teare, I.D.: Influence of high temperature on pollen grain viability and pollen tube growth in the styles ofPhaseolus vulgaris L. - J. amer. Soc. hort. Sci.105: 12–14, 1980.Google Scholar
- Larter, E., Chaubey, C.: Use of exogenous substances in promoting pollen tube growth and fertilization in barley-rye cross. - Can. I. Genet. Cytol.7: 511–518, 1965.Google Scholar
- Sabja, A.M., Mok, D.W.S., Mok, M.C.: Seed and embryo growth in food cultivars ofPhaseolus vulgaris andP. vulgaris×P. acutifolius. - Hort. Sci.25: 1288–1291, 1990.Google Scholar
- Singh, M., Khanna, V.K.: Effect of gamma radiations on the crossability of wheat, triticale and rye on meiosis, pollen grain germination and pollen tube growth. - Cytologia53: 123–130, 1988.Google Scholar