Biologia Plantarum

, 35:491 | Cite as

In vitro modification of spinach plasmalemma thickness

  • P. Crespi
  • M. Crèvecoeur
  • C. Penel
  • H. Greppin


Floral induction in the long day plant spinach (Spinacia oleracea) has been shown to be accompanied by a thickening of plasmalemma. This change was observed at early evocation, in both shoot apices and leaves, as well as after inducing GA3 treatment. To get further information on this thickening, plasma membranes from spinach leaves were isolated, in the present study, using aqueous two phase partitioning and the effect of variousin vitro treatments on their thickness was investigated. The average plasmalemma thickness was unaffected by Na+ and K+ ions. It was increased upon the effect of either Ca2+ or gibberellic acid. A thickening of plasmalemma was also observed when plasma membranes from vegetative plants were incubated with a cytosolic preparation from photoinduced plants. The results were discussed in relation with the plasmalemma modifications previously reported in spinach.


Gibberellin Shoot Apex Floral Induction Spinach Leave Spinacia Oleracea 
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  1. Auderset, G., Sandelius, A.S., Penel, C., Brightman, S.A., Greppin, H., Morré, D.J.: Isolation of plasma membrane and tonoplast fractions from spinach leaves by preparative free-flow electrophoresis and effect of photoinduction. - Physiol. Plant.68: 1–12, 1986.CrossRefGoogle Scholar
  2. Crespi, P., Crèvecoeur, M., Penel, C., Greppin, H.: Changes in plasmalemma after gibberellic acid treatment. - Plant Sci.62: 63–71, 1989.CrossRefGoogle Scholar
  3. Crespi, P., Crèvecoeur, M., Lefort, F., Greppin, H.: Modification of apical meristem plasmalemma during floral induction ofSpinacia oleracea (cv. Nobel). - Physiol. Plant.79(2, Part 2): A 19, 1990.Google Scholar
  4. Crèvecoeur, M., Deltour, R., Bronchart, R.: Quantitative freeze-fracture study of plasmalemma and nuclear envelope ofZea mays root cells during early germination. - J. Ultrastruct. Res.80: 1–11, 1982.PubMedCrossRefGoogle Scholar
  5. Crèvecoeur, M., Crespi, P., Lefort, F., Greppin, H.: Sterols and plasmalemma modifications in spinach apex during transition to flowering. - J. Plant Physiol.139: 595–599, 1992.Google Scholar
  6. Fontana, D.: Thèse No. 2098, Univ. Genève, Genève 1983.Google Scholar
  7. Friedman, H., Goldschmidt, E.E., Halevy, A.H.: Involvement of calcium in the photoperiodic flower induction process ofPharbitis nil. - Plant Physiol.89: 530–534, 1989.PubMedGoogle Scholar
  8. Gilmour, S.J., Zeevaart, J.A.D., Schwenen, L., Graeber, J.E.: The effect of photoperiod on gibberellin metabolism in cell free extract from spinach. - Plant Physiol.557: 92–95, 1985.Google Scholar
  9. Greppin, H., Auderset, G., Bonzon, M., Degli Agosti, R., Penel, C.: Flowering and leaf shoot interactions. - In: Wagner, E., Greppin, H., Millet, B. (ed.): The Cell Surface in Signal Transduction. Pp. 157–167, Springer - Verlag, Berlin 1987.Google Scholar
  10. Greppin, H., Bonzon, M., Crespi, P., Crèvecoeur, M., Degli Agosti, R., Penel, C.: Physiological macrofunctions and indicators of the flowering process. - In: Millet, B., Greppin, H. (ed.): Intraand Intercellular Communications in Plants. P. 107. INRA, Paris 1990.Google Scholar
  11. Havelange A.: Levels and ultrastructural localization of calcium inSinapis alba during the floral transition. - Plant Cell Physiol.30: 351–358, 1989.Google Scholar
  12. Hodges, T.K., Leonard, R.T.: Purification of a plasma membrane-bound adenosine triphosphatase from plant roots. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 32. Pp. 392–406. Academic Press, New York -San Francisco - London 1974.Google Scholar
  13. Kauss, H., Jeblick, W.: Activations by polyamines, polycations and ruthenium of the Ca2+-dependent glucan synthase from soybean cell. - FEBS Lett.185: 226–230. 1985.CrossRefGoogle Scholar
  14. Ladyzhenskaya, E.P., Dardzhaniya, L.G., Korableva, N.P.: [Influence of gibberellic and abscisic acid on some properties of the plasmalemma of potato tuber cells.] - Fiziol. Rast.34: 569–576, 1987. [In Russ.]Google Scholar
  15. Metzger, J.D., Zeevaart, J.M.: Effect of photoperiod on the levels of endogenous gibberellin in spinach as measured by combined gas chromatography selected ion current monitoring. - Plant Physiol.66: 844–846, 1980.PubMedGoogle Scholar
  16. Morré, D.J., Bracker, C.E.: Ultrastructural alternation in plant plasma membrane induced by auxin and calcium ions.- Plant Physiol.58: 544–547, 1976.PubMedGoogle Scholar
  17. Morré, D.J., Penel, C., Greppin, H.: Protoplasma, submitted, 1993.Google Scholar
  18. Murakami, S., Packer, L.: Light-induced changes in the conformation and configuration of the thylakoid membrane ofUlva andPorphyra chloroplastsin vivo. - Plant Physiol.45: 289–200, 1970.PubMedCrossRefGoogle Scholar
  19. Nelles, A.: Effect of gibberelic acid (GA3) on the relative ion permeability(PNa:Pk) of com coleoptile cells. - Biochem. Physiol. Pflanzen171: 349–351, 1977.Google Scholar
  20. Penel, C., Crespi, P., Crèvecoeur, M., Morré, D.J., Greppin, H.: Changes in plasma membrane sterols during floral induction. - In: Krekule, J., Seidlová, F. (ed.): Signals in Plant Development. Pp. 107–118. SPB Academic Publishing, The Hague 1989.Google Scholar
  21. Pharis, R.P., King, R.W.: Gibberellins and reproductive development in seeds plant. - Annu. Rev. Plant Physiol.36: 517–568, 1985.CrossRefGoogle Scholar
  22. Quail, PH.: Intracellular localization of phytochrome. - In: Trends in Photobiology. P. 485. Plenum Press, New York 1980.Google Scholar
  23. Rochester, C.P., Kjellbom, P., Larsson, C.: Lipid composition of plasma membranes from barley leaves and roots, spinach leaves and cauliflower inflorescences. - Physiol. Plant.71: 257–263, 1987.CrossRefGoogle Scholar
  24. Wang, Y., Leigh, R.A., Kaestner, K.H., Sze, H.: Electrogenic H+-pumping pyrophosphatase in tonoplast vesicles of oat roots. - Plant Physiol.81: 497–502, 1986.PubMedGoogle Scholar
  25. Wharton, D.C., Tzagaloff, A.: Cytochrome oxidase from beef heart mitochondria. - In: Colowick, S.P., Kaplan, NO. (ed): Methods in Enzymology. Vol. 10. Pp. 245–250. Academic Press, New York-London 1967.Google Scholar
  26. Wheeler, H., Baker, B.L.: Ultrastructure of thick membranes in plant roots exposed to uranyl and calcium salts. - J. Ultrastruct. Res.43: 355–361, 1973.PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Experimental Botany 1993

Authors and Affiliations

  • P. Crespi
    • 1
  • M. Crèvecoeur
    • 1
  • C. Penel
    • 1
  • H. Greppin
    • 1
  1. 1.Laboratory of Plant Biochemistry and Physiology, 3Place de I’UniversitéGenéve 4Switzerland

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