Folia Microbiologica

, Volume 30, Issue 6, pp 465–473 | Cite as

Effects of suspension density on microbial metabolic processes

  • S. Janda
  • A. Kotyk


Respiration of yeastsSaccharomyces cerevisiae, Bhodotorula glutinis, Endomyces magnusii, andCandida utilis, and of bacteriaEscherichia coli andBacillus megaterium, anaerobic production of CO2 byS. cerevisiae, active transport of quinovose byR, glutinis and ofL-proline andL-leucine byS. cerevisiae were highly dependent on cell suspension density. Respiration ofS. cerevisiae in the presence of glucose decreased in a biphasic fashion from 140 to 40 nmol O2 per mg dry solid per min as suspension density increased from 0.01 to 2 mg/mL. Higher partial pressures of oxygen further enhanced the trend. The active transports were affected monophasically in the maximum rate of uptake which was as much as ten times greater at low than at high suspension densities. A component of the external medium is suspected to cause the decrease of metabolic functions at higher cell densities, acting as a noncompetitive inhibitor. The component was present and mutually active in suspensions of the various yeasts as wellas of bacteria. Its properties and results of model experiments suggest it to be dissolved carbon dioxide.


Bacillus Megaterium Candida Utilis High Partial Pressure Suspension Density Dissolve Carbon Dioxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cohn M., Monod J.: Purification et propriétés de la β-galactosidase (lactase)d’Escherichia coli.Biochim.Biophys.Acta 7, 153 (1951).PubMedCrossRefGoogle Scholar
  2. Dixon M.: The determination of enzyme inhibition constants.Biochern. J. 55, 170 (1953).Google Scholar
  3. Eddy A.A.: Proton-dependent solute transport in microorganisms.Gurr.Topics Membr.Transport 10, 83 (1978).Google Scholar
  4. Hobák J., Kotyk A.: Energetics ofL-proline transport in baker’s yeast.Biochim.Biophys.Acta, in press (1986).Google Scholar
  5. Hobák J., Říhová L.: L-Proline transport inSaccharomyces cerevisiae.Biochim.Biophys.Acta 691, 144 (1982).CrossRefGoogle Scholar
  6. Janda S.: Relationship of active membrane transport and respiration inRhodotorula glutinis: possibility of two respiratory systems.Cell.Molec.Biol. 25, 131 (1979).Google Scholar
  7. Jones B.P., Gbeenfikld P.F.: Effect of carbon dioxide on yeast growth and fermentation.Enzyme Microbiol.Technol. 4, 210 (1982).CrossRefGoogle Scholar
  8. Kotyk A., Höfer M.: Uphill transport of sugars in the yeastRhodotorula gracilis.Biochim.Biophys.Acta 102, 410 (1965).PubMedCrossRefGoogle Scholar
  9. Kotyk A., Haškovec C.: Properties of the sugar carrier in baker’s yeast. III. Induction of the galactose carrier.Fo ia Microbiol. 13, 12 (1968).CrossRefGoogle Scholar
  10. KotykA., Miohaijaničová D., Vereš K., Soukupová V.: Transport of 4-deoxy- and 6-deoxy-D- glucose in baker’s yeast.Folia Microbiol. 20, 496 (1975).CrossRefGoogle Scholar
  11. Kotyk A., MichaIiJaničová D.: Uptake of trehalose bySaccharomyces cerevisiae.J.Gen. Microbiol. 110, 323 (1979).PubMedGoogle Scholar
  12. McQuillen K.: Bacterial protoplasts. I. Protein and nucleic acid metabolism in protoplasts ofBacillus megaterium. Biochim.Biophys.Acta 17, 382 (1955).PubMedCrossRefGoogle Scholar
  13. Michaljaničová D., Kotyk A.: pH dependence of 6-deoxy-D-glucose uptake in different yeast genera.12th FEBS Meeting, Abstract 1413; Dresden 1978.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1985

Authors and Affiliations

  • S. Janda
    • 1
  • A. Kotyk
    • 1
  1. 1.Department of Cell Physiology, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

Personalised recommendations