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Folia Microbiologica

, Volume 26, Issue 3, pp 184–188 | Cite as

Methionine-defective mutants inCandida utilis

  • J. M. Delgado
  • A. Alonso
  • X. Alvarez
  • L. S. Herrera
Article

Abstract

Ethionine-resistant mutants ofCandida utilis CCY-158 overproducing methionine have been isolated. In these mutants the intracellular methionine concentration decreased significantly during the stationary phase. The wild-type strain CCY-158 and the ethionine-resistant mutants isolated were able to use methionine as the nitrogen source but not as the carbon source. From these ethionine-resistant mutants we isolated mutants unable to use methionine as nitrogen source (Mec- mutants), the principal alteration being at the level of methionine uptake. Some of the Mec mutants lost also the ability to use other amino acids as nitrogen source.

Keywords

Methionine Nitrogen Source Original Strain Ethionine Candida Utilis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1981

Authors and Affiliations

  • J. M. Delgado
    • 1
  • A. Alonso
    • 1
  • X. Alvarez
    • 1
  • L. S. Herrera
    • 1
  1. 1.Department of Genetics and Biochemistry of MicroorganismsCentro Nacional de Investigaciones CientificasLa HabanaCuba

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