Folia Microbiologica

, Volume 26, Issue 5, pp 426–429 | Cite as

Correction method for estimating the number of reversions in bacteria

  • E. Říhová
Brief reports


A correction of the results of mutation tests in bacteria during the induction of reversions from auxotrophy to prototrophy is described. The correction is based on a calibration curve of the spontaneous frequency of reversions and is suitable for testing doses of mutagens that exhibit a pronounced lethal effect.


AITC Ethyl Methanesulfonate Allylisothiocyanate Selective Plate Revertant Coloni 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ames B.N., Durston W.E., Yamasaki E., Lee F.D.: Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection.Proc.Nat.Acad.Sci.USA 70, 2281 (1973).PubMedCrossRefGoogle Scholar
  2. Ellenbergeb J., Mohn G.: Mutagenic activity of cyclophosphamide, ifosfamide and trofosfamide in different genes ofEscherichia coli andSalmonella typhimurium after biotransformation through extracts of rodent liver.Arch.Toxicol. 33, 225 (1975).CrossRefGoogle Scholar
  3. Green M.H.L., Muriel W.J.: Mutagen testing usingtrp + reversion inEscherichia coli.Mutation Bes. 38, 3 (1976).Google Scholar
  4. de Mitchell G.I.: Microbial assays for mutagenicity: A modified liquid culture method compared with the agar plate system for precision and sensitivity.Mutation Bes. 54, 1 (1978).Google Scholar
  5. Mohn G., Ellenberger J., Mc Gregor D., Merker H.J.: Mutations in plants, microorganisms and in host-mediated assay systems.Mutation Bes. 29, 221 (1975).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1981

Authors and Affiliations

  • E. Říhová
    • 1
  1. 1.Department of Genetics, Faculty of SciencesCharles UniversityPrague 2

Personalised recommendations