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Inhibitory Action of Pig Alpha-Fetoprotein Early Activated Thymocyte Surface trohugh Na+,K+-Adenosinetriphosphatase

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Abstract

An immunomodulatory effect of pig alpha-fetoprotein (AFP) on early phytohaemagglutinin (PHA)-stimulated intact thymocytes from piglets was analyzed. The activity of surface-localized Na+, K+-adenosinetriphosphata sewas estimated, as the early reaction of mitogen-stimulated T-lymphocytes. A high inhibition of PHA-stimulated enzyme activity was found in the presence of AFP. The reaction was dependent on PHA concentration when using a constant AFP concentration. The highest AFP-induced decrease in PHA-induced Na+, K+-ATPase activity was 72 %. AFP was isolated by a new technique based on salting out in ammonium sulphate and acetate buffer solutions. A gentle, simple and rapid procedure makes it possible to obtain AFP in a high degree of purity with preserved biological activity. The presented results can contribute to understanding the molecular aspects of AFP immunoregulation of lymphocyte reactivityin vitro.

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  1. Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    H. Kovářů & F. Kovářů

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  1. H. Kovářů
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  2. F. Kovářů
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Kovářů, H., Kovářů, F. Inhibitory Action of Pig Alpha-Fetoprotein Early Activated Thymocyte Surface trohugh Na+,K+-Adenosinetriphosphatase. Folia Microbiol 26, 417–421 (1981). https://doi.org/10.1007/BF02927338

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