Abstract
The production of CM and FP cellulases was studied during the growth of a wild strain ofTrichoderma viride on microcrystalline cellulose. Part of the enzymes was found to be released into the medium while another part remained bound to the cell. Bound cellulases are released into the medium at the stage of cell lysis which takes place in the post-stationary phase. In this period extracellular CM and FP cellulases attain maximum activities. When the hyphae are subjected to a cold shock, maximum cellulase activity is detected already at the beginning of the stationary phase. An indirect method of dry cell mass determination showed that during exponential growth of cells on microcrystalline cellulose the μmax was 0.23 and the yield coefficient was 41 %.
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References
Berg B., Hofsten A.V.: The ultrastructure of the fungusTrichoderma viride and investigation of its growth on cellulose.J.Appl.Bacteriol.41, 395 (1976).
Berg B., Pettersson G.: Location and formation of cellulases inTrichoderma viride.J.Appl.Bacteriol.42, 65 (1977).
Binder A., Ghose T.K.: Adsorption of cellulose byTrichoderma viride.Biotechnol.Bioeng.20, 1187 (1978).
Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F.: Oolorimetric method for determination of sugars and related substances.Anal.Chem.28, 350 (1956).
Ghose T.K.: Cellulase biosynthesis and hydrolysis of cellulosic substances.Adv.Biochem.6,39 (1977).
Hakansson U., Fägerstam L., Pettersson G., Andersson L.: Purification and characterization of a low molecular weight 1,4-β-glucan glucanohydrolase from the cellulolytic fungusTrichoderma viride QM 9419.Biochim.Biophys.Acta524, 385 (1978).
Linko M.: An evaluation of enzymatic hydrolysis of cellulosic materials.Adv.Biochem.Eng.5, 25 (1977).
Lowry O.H., Rosebrough N.J., Farr N.J., Randall R.J.: Protein measurement with the Folin phenol reagent.J.Biol.Chem.193, 265 (1951).
Mandels M., Andreotti R.E.: Problems and challenges in the cellulose to cellulase fermentation.Process Biochem.13, 6 (1978).
Mandels M., Sternberg D.: Recent advances in cellulase technology.J. Ferment. Technol.54, 267 (1976).
Mandels M., Andreotti R., Roche C.: Measurement of saccharifying cellulase.Biotechnol. Bioeng. Symp.6, 21 (1976).
Mandels M., Weber J.: The production of cellulasesJ. Adv. Chem. Ser.95, 391 (1969).
Miller G.L.: Use of dinitrosalicylic acid reagent for determination of reducing sugar.Anal.Chem.31, 426 (1959).
Montenecourt B.S., Eveleigh D.E.: Preparation of mutants ofTrichoderma reesei with enhanced cellulase production.Appl.Env.Microbiol.34. 777 (1977).
Nystrom J.M., Allen A.L.: Pilot scale investigations and economics of cellulase production.Biotechnol. Bioeng. Symp.6. 55 (1976).
Okada G.: Enzymatic studies on a cellulase system ofTrichoderma viride. IV. Purification and properties of a less-random type cellulase.J.Biochem.80, 913 (1976).
Peitersen N.: Continuous cultivation ofTrichoderma viride on cellulose.Biotechnol.Bioeng.19, 337 (1977).
Pettersson L.G.: The mechanism of enzymatic hydrolysis of cellulose byTrichoderma viride. Proc.SITRA Symp. Enzymatic Hydrolysis of Cellulose (Aulanko, Helsinki) 255 (1975).
Sternberg D.: A method for increasing cellulase production byTrichoderma viride.Biotechnol.Bioeng.18, 1751 (1976).
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Kyslíková, E., Volfová, O. Cell growth and cellulase production inTrichoderma viride on microcrystalline cellulose. Folia Microbiol 26, 303–308 (1981). https://doi.org/10.1007/BF02927256
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DOI: https://doi.org/10.1007/BF02927256