Use of nalidixic acid for constructing the replication map of theMycobacterium phlei chromosome
Nalidixic acid was used for describing more accurately the terminal replication region of theMycobacterium phlei chromosome. Cell division in synchronized cultures was not sensitive to this acid any more between 185–190 min,i.e. about 10 min after replication of theser gene the last of 24 genes of the replication map described so far. The replication of the chromosome was controlled by determining the position of thebac gene. Microscopic studies in phase contrast of the cells that were subjected for long time periods to nalidixic acid treatment at a bactericidal concentration showed elongated cells. The electron-microscopic observation showed that a portion of the population influenced by nalidixic acid lyzes, whereas other cells remain intact and resemble control cells.
KeywordsNalidixic Acid Neisseria Meningitidis Auxotrophic Mutant Chromosome Replication Replication Point
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