Folia Microbiologica

, Volume 26, Issue 1, pp 65–67 | Cite as

Use of ruthenium red for visualization of the regenerating cell wall in yeast protoplasts

  • M. Havelková


Ruthenium red was used to stain the surface of fresh And regenerating protoplasts inSacceharomyses cerevisine. The presence of a dense layer, 10–40 mm wide, was demonstrated on the surface of most of the fresh protoplasts. This layer could be removed neither by repeated washings of protoplasts nor by their centrifugation. Ruthenium red proved to be a very useful dye for regenerating walls since it stained both the amorphous and the fibrillar components, but to a different degree, thus permitting their easy differentiation.


Osmium Tetroxide Dense Layer Regenerate Cell Wall Repeated Washing Tetro 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1981

Authors and Affiliations

  • M. Havelková
    • 1
  1. 1.Department of Biology, Faculty of MedicineJ.E. Purkyně, UniversityBrnoCzechoslovakia

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