Use of ruthenium red for visualization of the regenerating cell wall in yeast protoplasts
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Ruthenium red was used to stain the surface of fresh And regenerating protoplasts inSacceharomyses cerevisine. The presence of a dense layer, 10–40 mm wide, was demonstrated on the surface of most of the fresh protoplasts. This layer could be removed neither by repeated washings of protoplasts nor by their centrifugation. Ruthenium red proved to be a very useful dye for regenerating walls since it stained both the amorphous and the fibrillar components, but to a different degree, thus permitting their easy differentiation.
KeywordsOsmium Tetroxide Dense Layer Regenerate Cell Wall Repeated Washing Tetro
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- Havelková M.: Fixation of yeast cells and protoplasts for electron microscopy. (In Czech) Reports of the Commision of Electron Microscopists of the Slovak Biological Society2, 6 (1968).Google Scholar
- Havelková M.: Ultrastructure of freshly prepared, growing and reverting protoplasts of various yeast species. (In Czech)Dissertation thesis. Department of Biology, Medical Faculty, J.E. Purkyně University, Brno 1974.Google Scholar
- Jensen W.A.:Botanical Histochemistry. Principles and Practice. W.H. Freeman & Co., San Francisco 1962.Google Scholar
- Kopp F.: Electron microscopy of yeasts, p. 37 inMethods in Cell Biology D.M. Prescott, Ed., Vol. XI. Academic Press. New York-San Francisco-London 1975.Google Scholar
- Mangin L.: Sur l’emploi du rouge de ruthénium en anatomic végétale.Compt.Rend.116, 653 (1893).Google Scholar
- Nečas O.: The mechanism of regeneration of yeast protoplasts. II. Formation of the cell wallde novo.Folia Biol.11, 97 1965.Google Scholar