Use of ruthenium red for visualization of the regenerating cell wall in yeast protoplasts
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Ruthenium red was used to stain the surface of fresh And regenerating protoplasts inSacceharomyses cerevisine. The presence of a dense layer, 10–40 mm wide, was demonstrated on the surface of most of the fresh protoplasts. This layer could be removed neither by repeated washings of protoplasts nor by their centrifugation. Ruthenium red proved to be a very useful dye for regenerating walls since it stained both the amorphous and the fibrillar components, but to a different degree, thus permitting their easy differentiation.
KeywordsOsmium Tetroxide Dense Layer Regenerate Cell Wall Repeated Washing Tetro
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