Skip to main content
SpringerLink
Log in

Physiological characterictics and energy balance ofKlebsiella aerogenes in a multistage tower fermentor

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Biomass growth, consumption of carbon and energy source, specific rates of formation of metabolic byproducts, biomass yield referred to the C-source and to oxygen, respiration rate and the value ofRQ were studied inKlebsiella aerogenes CCM 2318 (on a synthetic glucose medium) at different specific growth rates. Maintenance coefficients and the total energy balance of the cultivation process were evaluated for a multistage tower fermentor with a defined interstage mixing. The results pointed to changes in both glucose metabolism and the physiological state of the population, brought about by changes in specific growth rate. As compared with a chemostat, the culture was found to exhibit a different physiological character in stages 1 and 4 despite a considerable interstage mixing.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akiba T., Fukimbara T.: Fermentation of volatile substrate in a tower-type fermenter with a gas entrainment process.J. Ferment. Technol.51, 134 (1973).

    CAS  Google Scholar 

  • Bolton P. G., Rotgers P. J., Dean A. C. R.: Molar growth yields and acid phosphatase synthesis inKlebsiella aerogenes growing in carbon-limited chemostat culture.J. Appl. Chem. Biotechnol.22, 941 (1972).

    Article  CAS  Google Scholar 

  • Camabgo-Bubio E., Ornelas-Vale A., Cassarubias-Arcos G., Nagai S.: Energetic balance and cellular composition ofCandida utilis in sugar-limited chemostat cultures based on nopal fruit.J. Ferment. Technol.55, 56 (1977).

    Google Scholar 

  • Conway E. J.:Microdiffusion Analysis and Volumetric Error, 3rd Ed. Crocby Lockwood Sons, London 1950.

    Google Scholar 

  • Degn H., Lilleør M., Lønsmann Iversen J. J.: The occurrence of a step wise-decreasing respiration rate during oxidative assimilation of different substrates by restingKlebsiella aerogenes in a system open to oxygen.Biochem. J.136, 1097 (1973).

    PubMed  CAS  Google Scholar 

  • Doelle H. W., Hollywood N., Westwood A. W.: Effect of glucose concentration on a number of enzymes involved in the aerobic and anaerobic utilization of glucose in turbidostat cultures ofEscherichia coli.Microbios9, 221 (1974).

    PubMed  CAS  Google Scholar 

  • Doležal J., Kapbálek F.: Physiological characteristics of the chemostatically grownCitrobacter freundii as a function of the specific growth rate and type of nutrient limitation.Folia Microbiol.21, 168 (1976).

    Article  Google Scholar 

  • Falch E. A., Gaden E. L.: A continuous multistage tower fermentor. I. Design and performance tests.Biotechnol. Bioeng.11, 927 (1969).

    Article  Google Scholar 

  • Falch E. A., Gaden E. L.: A continuous multistage tower fermentor. II. Analysis of reactor performance.Biotechnol. Bioeng.12, 465 (1970).

    Article  Google Scholar 

  • Goto S., Kitai A., Ozaki A.: Continuous yeast cell production from ethanol with a multi-stage tower fermentor.J. Ferment. Technol.51, 582 (1973).

    CAS  Google Scholar 

  • Grégr V., Rychtera M.:Analytical Methods to Practical Exercises in Fermentation Chemistry and Technology (in Czech), Vol. III. SNTL, Prague 1966.

    Google Scholar 

  • Hadjipetrou L. P., Gerrits J. P., Teulings F. A. G., Stouthamer A. H.: Relation between energy production and growth ofAerobacter aerogenes.J. Gen. Microbiol.36, 139 (1964).

    CAS  Google Scholar 

  • Hadjipetrou L. P., Stottthamer A. H.: Energy production during nitrate respiration byAerobacter aerogenes.J. Gen. Microbiol.38, 29 (1965).

    PubMed  CAS  Google Scholar 

  • Harrison D. E. F., Loveless J. E.: The effect of growth condition on respiratory activity and growth efficiency in facultative anaerobes grown in chemostat culture.J. Gen. Microbiol.68, 35 (1971a).

    PubMed  CAS  Google Scholar 

  • Harrison D. E. F., Loveless J. E.: Transient responses of facultatively anaerobic bacteria growing in chemostat culture to a change from anaerobic to aerobic conditions.J. Gen. Microbiol.68, 45 (19716).

    PubMed  CAS  Google Scholar 

  • Herbert D., Elsworth R., Telling R. C.: The continuous culture of bacteria: theoretical and experimental study.J. Gen. Microbiol.14, 601 (1956).

    PubMed  CAS  Google Scholar 

  • Herbert D.: Continuous culture of microorganisms. Some theoretical aspects, p. 45 inContinuous Cultivation of Microorganisms. Publ. House Czech. Acad. Sci., Prague 1958.

    Google Scholar 

  • Hobson P. N., Summers R.: The continuous culture of aerobic bacteria.J. Gen. Microbiol.47, 53 (1967).

    PubMed  CAS  Google Scholar 

  • Hollywood N., Doelle H. W.: Effect of specific growth rate and glucose concentration on growth and glucose metabolism ofEscherichia coli K-12.Microbios17, 23 (1976).

    PubMed  CAS  Google Scholar 

  • Hsu K. H., Erickson L. E., Fan L. T.: Oxygen transfer to mixed cultures in tower systems.Biotechnol. Bioeng.17, 499 (1975).

    Article  CAS  Google Scholar 

  • Hsu K. H., Erickson L. E., Fan L. T.: Pressure drop, gas hold-up and oxygen transfer in tower systems.Biotechnol. Bioeng.19, 247 (1977).

    Article  CAS  Google Scholar 

  • Kitai A., Goto S., Ozaki A.: The performance of a perforate plate column as a multistage continuous fermentor. II. Flow characteristics in cocurrent air-liquid system.J. Ferment. Technol.47, 340 (1969a).

    CAS  Google Scholar 

  • Kitai A., Goto S., Ozaki A.: The performance of perforated plate column as a multistage continuous fermentor. III. Determination of oxygen transfer coefficient.J. Ferment. Technol.47, 348 (1969b).

    CAS  Google Scholar 

  • Kitai A., Tone H., Ozaki A.: Performance of a perforated plate column as a multistage continuous fermentor.Biotechnol. Bioeng.11, 911 (1969c).

    Article  Google Scholar 

  • Kitai A., Yamagata T.: Perforated plate column fermentor.Proc. Biochem.5, 52 (1970).

    CAS  Google Scholar 

  • Kitai A.,Okamoto R.,Ozaki A.: Continuous culture using a perforated plate column. Paper at 4th Internat. Ferment. Symp., Tokyo 1972.

  • Knook D. L., Van Riet J., Planta R. J.: The participation of cytochtomes in the process of nitrate respiration inKlebsiella (Aerobacter) aerogenes.Biochim. Biophys. Acta292, 237 (1973).

    Article  CAS  Google Scholar 

  • Lehninger A. L.:Biochemistry, p. 980. Worth Publishers, New York 1975.

    Google Scholar 

  • Mayberry W. R., Pbochazka G. L., Payne W. J.: Factors derived from studies of aerobic growth in minimal media.J. Bacteriol.96, 1424 (1968).

    PubMed  CAS  Google Scholar 

  • Mayer D. J., Jones C. W.: Reactivity with oxygen of bacterial cytochtome oxidases ai, aa3 and o.FEBS Letters33, 101 (1973a).

    Article  Google Scholar 

  • Mayer D. J., Jones C. W.: Oxidative phosphorylation in bacteria which contain different cytochrome oxidases.Eur. J. Biochem.36, 144 (1973b).

    Article  Google Scholar 

  • Morris G. G., Greenshields B. N., Smith E. L.: Aeration in tower fermenters containing microorganisms, p. 535 inAdvances in Microbial Engineering, Part 1 (B. Sikyta, A. Prokop, M. Novák, Eds). Wiley & Sons, New York 1973.

    Google Scholar 

  • Neidhardt F. C.: Role of enzyme repression in the regulation of catabolism in bacteria. Colloque International du Centre National de la Recherche Scientifique124, 329 (1963).

    Google Scholar 

  • Neidhardt F. C.: Méchanismes de régulation des activités cellulaires chez les microorganismes.Centre Natl. Reck. Sci. Symp. (Paris)124, 329 (1965).

    Google Scholar 

  • Nishizawa Y., Nagai S., Aiba S.: Grown yields ofRhodopseudomonas spheroides in dark and aerobic chemostat cultures.J. Ferment. Technol.52, 526 (1974).

    CAS  Google Scholar 

  • Páaca J.: Effect of oxygen supply on the course of batch cultivations of facultatively anaerobic bacteria.Sci. Papers Inst. Chem. Technol. (Prague)E 43, 67 (1975).

    Google Scholar 

  • Páca J.: Oxygen transfer rate, respiration and yields in batch and chemostat cultures ofKlebsiella aerogenes.Folia Microbiol.21, 417 (1976).

    Article  Google Scholar 

  • Páca J.: Effect of oxygen supply on growth ofKlebsiella aerogenes in a multistage tower fermentor.Folia Microbiol.23, 108 (1978).

    Article  Google Scholar 

  • Páca J., Grégr V.: Design and performance characteristics of a continuous multistage tower fermentor.Biotechnol. Bioeng.18, 1075 (1976).

    Article  Google Scholar 

  • Páca J., Grégr V.: Effect of viscosity on backflow coefficient and oxygen transfer rate in a multistage tower fermentor.J. Ferment. Technol.55, 166 (1977a).

    Google Scholar 

  • Páca J., Grégr V.: Growth characteristics ofCandida utilis on volatile substrate in a multistage tower fermentor.Biotechnol. Bioeng.19, 539 (1977b).

    Article  PubMed  Google Scholar 

  • Páca J., Grégr V.: Growth and metabolic activity ofKlebsiella aerogenes in a chemostat culture.J. Ferment. Technol.55, 213 (1977c).

    Google Scholar 

  • Pannell S. D., Greenshields R. N.: A tower fermenter design for the continuous culture of filamentous fungi. Paper at Symp. FEMS, Vienna 1977.

    Google Scholar 

  • Payne W. J.: Energy yields and growth of heterothrophs.Ann. Rev. Microbiol.24, 17 (1970).

    Article  CAS  Google Scholar 

  • Pirt S. J.: The maintenance energy of bacteria in growing cultures.Proc. Roy. Soc. London, Ser.B 163. 224 (1965).

    Article  Google Scholar 

  • Pirt S. J.:Principles of Microbe and Cell Cultivation, p. 68. Blackwell Scientific Publications, Oxford 1975.

    Google Scholar 

  • Prokop A., Erickson L. E., Fernandez J., Humphrey A. E.: Design and physical characteristics of a multistage, continuous tower fermentor.Biotechnol. Bioeng.11, 945 (1969).

    Article  Google Scholar 

  • Stouthamer A. H., BettenhausenC.: Influence of hydrogen acceptors on growth and energy production ofProteus mirabilis.Antonie van Leeuwenhoek38, 81 (1972).

    Article  PubMed  CAS  Google Scholar 

  • Stouthamer A. H., Bettenhausen C.: Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms.Biochim. Biophys. Acta301, 53 (1973).

    PubMed  CAS  Google Scholar 

  • Stouthamer A. H.:Yields Studies in Microorganisms, p. 17. Meadowfield Press, Durham 1976.

    Google Scholar 

  • Todt J., Lücke J., Schügerl K., Renken A.: Gas holdup and longitudinal dispersion in different types of multiphase reactors aad their possible application for microbial processes.Chem. Eng. Sci.32, 369 (1977).

    Article  CAS  Google Scholar 

  • Veldkamp H.:Continuous Culture in Microbial Physiology and Ecology, p. 32. Meadowfield Press, Durham 1976.

    Google Scholar 

  • De Vries W., Kaptejn M. C., Van Der Beek E. G., Stouthamer A. H.: Molar growth yields and fermentation balances ofLactobacillus casei L 3 in batch cultures and in continuous cultures.J. Gen. Microbiol.63, 333 (1970).

    PubMed  Google Scholar 

  • Wimpenny J. W. T., Necklen D. K.: The redox environment and microbial physiology. I. The transition from anaerobiosis to aerobiosis in continuous cultures of facultative anaerobes.Biochim. Biophys. Acta253, 352 (1971).

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Fermentation Chemistry and Technology, Institute of Chemical Technology, 166 28, Prague 6

    J. Páca

Authors
  1. J. Páca
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Páca, J. Physiological characterictics and energy balance ofKlebsiella aerogenes in a multistage tower fermentor. Folia Microbiol 24, 352–363 (1979). https://doi.org/10.1007/BF02926656

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02926656

Keywords

Search

Navigation