Biologia Plantarum

, 35:179 | Cite as

Some factors affecting somatic embryogenesis efficiency in soybean (Glycine max (L.) Merr.)

  • M. Griga


Selected factors affecting somatic embryogenesis efficiency have been studied, namely genotype, explant type and its orientation in the medium, different basal media, different auxins for somatic embryo induction, and two ways of donor plant cultivation. The key role is played by genotype and auxin used, the minimum effect was observed due to basal media. In the series of subsequent experiments we have found the best combination of individual factors as follows: cv. Altona, 10 uM 2,4-D, L2 basal medium, central part of immature cotyledon as initial expiant oriented by adaxial side down on the agar medium, and field grown donor plants. This combination exhibited 100 % embryogenic explants with 5.43 ± 0.65 somatic embryos per expiant,i.e. somatic embryogenesis efficiency 5.43.


Somatic Embryo Somatic Embryogenesis Zygotic Embryo Picloram Dicamba 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



4-amino-3,5,6-trichloropicolinic acid-2,4-D-2,4-dichlorophenoxyacetic acid


α-naphthaleneacetic acid


3,6-dichloro-o-anisic acid


medium after Phillips and Collins 1979


medium with mineral salts after Murashige and Skoog 1962, vitamins after Gamborget al. 1968,


somatic embryogenesis


somatic embryogenesis efficiency


  1. Barwale, U.B., Kerns, H.R., Widholm, J.M.: Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta167: 473–481, 1986.CrossRefGoogle Scholar
  2. Barwale, U.B., Widholm, J.M.: Somaclonal variation in plants regenerated from cultures of soybean. Plant Cell Rep.6: 365–368, 1987.CrossRefGoogle Scholar
  3. Barwale, U.B., Widholm, J.M.: Soybean: Plant regeneration and somaclonal variation. In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry10. Pp.114–133. Springer-Verlag, Berlin 1990.Google Scholar
  4. Buchheim, J.A., Colburn, S.M., Ranch, J.P.: Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol.89: 768–775, 1989.PubMedCrossRefGoogle Scholar
  5. Christianson, M.L., Warnick, D.A., Carlson, P.S.: A morphogenetically competent soybean suspension culture. Science222: 632–634, 1983.PubMedCrossRefGoogle Scholar
  6. Christou, P., Yang, N.S.: Developmental aspects of soybean (Glycine max) somatic embryogenesis. Ann. Bot.64: 225–234, 1989.Google Scholar
  7. Finer, J.J.: Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]. Plant Cell Rep.7: 238–241, 1988.CrossRefGoogle Scholar
  8. Finer, J.J., Nagasawa, A.: Development of an embryogenic suspension culture of soybean (Glycine max Merrill). Plant Cell Tissue Organ Cult.15: 125–136, 1988.CrossRefGoogle Scholar
  9. Gamborg, O.L., Miller, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res.50: 151–158, 1968.PubMedCrossRefGoogle Scholar
  10. Ghazi, T.D., Cheema, H.V., Nabors, M.W.: Somatic embryogenesis and plant regeneration from embryogenic callus of soybean,Glycine max L. Plant Cell Rep.5: 452–456, 1986.CrossRefGoogle Scholar
  11. Griga, M.: [The study of regeneration systemsin vitro in pea, faba bean and soybean] Ph.D.- Thesis, Masaryk University, Brno 1990. [In Czech]Google Scholar
  12. Griga, M., Kubaláková, M., Tejklová, E.: Somatic embryogenesis inVicia faba. Plant Cell Tissue Organ Cult.9: 167–171, 1987.CrossRefGoogle Scholar
  13. Hartweck, L.M., Lazzeri, P.A., Cui, D., Collins, G.B., Williams, E.G.: Auxin-orientation effects on somatic embryogenesis from immature soybean cotyledons.In Vitro Cell Dev. Biol.24: 821–828, 1988.CrossRefGoogle Scholar
  14. Hazra, S., Sathaye, S.S., Mascarenhas, A.F.: Direct somatic embryogenesis in peanut(Arachis hypogaea). Biotechnology7: 949–951, 1989.CrossRefGoogle Scholar
  15. Hepher, A., Boulter, M.E., Harris N., Nelson, R.S.: Development of a superficial meristem during somatic embryogenesis from immature cotyledons of soybean (Glycine max L.). Ann. Bot.62: 513–519, 1988.Google Scholar
  16. Hildebrand, D.F., Adams, T.R., Dahmer, M.L., Williams, E.G., Collins, G.B.: Analysis of lipid composition and morphological characteristics in soybean regenerants. Plant Cell Rep.7: 701–703, 1989.Google Scholar
  17. Hinchee, M.A.W., Finer, J.J., Newell. CA., Sato, S.J., Tredo, O.L.: Soybean somatic embryogenesis as an avenue for transformation via particle bombardment. - In: Abstracts VIIth IAPTC Congress, Amsterdam. P. 60. 1990.Google Scholar
  18. Komatsuda, T., Ko, W.: Screening of soybean [Glycine max (L.) Merrill] genotypes for somatic embryo production from immature embryo. Jap. J. Breed.40: 249–251, 1990.Google Scholar
  19. Komatsuda, T., Ohyama, K.: Genotypes of high competence for somatic embryogenesis and plant regeneration in soybeanGlycine max. Theor. appl. Genet.75: 695–700, 1988.CrossRefGoogle Scholar
  20. Kysely, W., Jacobsen, H.J.: Somatic embryogenesis from pea embryos and shoot apices. Plant Cell Tissue Organ Cult.20: 7–14, 1990.CrossRefGoogle Scholar
  21. Kysely, W., Myers, J.R., Lazzeri, P.A., Collins, G.B., Jacobsen, H.J.: Plant regeneration via somatic embryogenesis in pea (Pisum sativum L.). Plant Cell Rep.6: 305–308, 1987.CrossRefGoogle Scholar
  22. Lazzeri, P.A., Hildebrand, D.F., Collins, G.B.: A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant mol. Biol. Rep.3: 160–167, 1985.CrossRefGoogle Scholar
  23. Lazzeri, P.A., Hildebrand, D.F., Collins, G.B.: Soybean somatic ebryogenesis: Effects of hormones and culture manipulations. Plant Cell Tissue Organ Cult.10: 197–208, 1987a.CrossRefGoogle Scholar
  24. Lazzeri, P.A., Hildebrand, D.F., Collins, G.B.: Soybean somatic embryogenesis: Effects of nutritional, physical and chemical factors. Plant Cell Tissue Organ Cult.10: 209–220, 1987b.CrossRefGoogle Scholar
  25. Lazzeri, P.A., Hildebrand, D.F., Sunega, J., Williams, E.G., Collins, G.B.: Soybean somatic embryogenesis: Interactions between sucrose and auxin. Plant Cell Rep.7: 517–520, 1988.CrossRefGoogle Scholar
  26. Lehminger-Mertens, R., Jacobsen, H.J.: Plant regeneration from pea protoplasts via somatic embryogenesis. Plant Cell Rep.8: 379–382, 1989.CrossRefGoogle Scholar
  27. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 472–497, 1962.CrossRefGoogle Scholar
  28. Novak, M., Griga, M., Tejklová, E.: Induction of somatic embryos from immature zygotic embryos ofGlycine max (L.) vitro. Sci. agr. Bohem.19: 233–241, 1987.Google Scholar
  29. Ozias-Akins, P.: Plant regeneration from immature embryos of peanut. Plant Cell Rep.8: 217–218, 1989.CrossRefGoogle Scholar
  30. Parrott, W.A., Williams, E.G., Hildebrand, D.F., Collins, G.B.: Effect of genotype on somatic embryogenesis from immature cotyledons of soybean. Plant Cell Tissue Organ Cult.16: 15–21, 1989.CrossRefGoogle Scholar
  31. Phillips, G.C., Collins, G.B.:In vitro tissue culture of selected legumes and plant regeneration from callus cultures of red clover. Crop Sci.19: 59–64, 1979.Google Scholar
  32. Ranch, J.P., Oglesby, L., Zielinski, A.C.: Plant regeneration from embryo-derived tissue cultures of soybean.-In Vitro Cell. Dev. Biol.21: 653–658, 1985.CrossRefGoogle Scholar
  33. Ranch, J.P., Oglesby, L., Zielinski, A.C.: Plant regeneration from tissue cultures of soybean by somatic embryogenesis. In: Vasil, I.K. (ed.): Cell Culture and Somatic Cell Genetics of Plants, Vol. 3. Pp. 97 - 110. Academic Press, Orlando 1986.Google Scholar
  34. Stejskal, J., Griga, M.: Somatic embryogenesis and plant regeneration inPisum sativum L. Biol. Plant.34: 15–22, 1991Google Scholar
  35. Tétu, T., Sangvan-Norreel, B., Sangvan, R.: Embryogénèse somatique et régénérationin vitro chez trois variétés précoces de Soja. Compt. rend. Acad. Sci., Sér. III.305: 613–617, 1987.Google Scholar

Copyright information

© Institute of Experimental Botany 1993

Authors and Affiliations

  • M. Griga
    • 1
  1. 1.Research Institute of Technical Crops and LegumesOsevaŠumperkCzech Republic

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