Biologia Plantarum

, 34:159 | Cite as

Effect of nacl- salinity on metabolism of proline in salt- sensitive and salt- resistant cultivars of rice

  • D. Roy
  • A. Bhunia
  • N. Basu
  • S. K. Banerjee
Brief communication


The effect of NaCl at sublethal concentration was observed on germinating seeds of salt-sensitive and -resistant rice cultivars with respect to the level of proline regulatory enzymes and the growth of seedlings on different days of early germination period. The two enzymes of proline biosynthesis and catabolism, Δ-pyrroline-5-carboxylate reductase and L-proline dehydrogenase, were taken into consideration to observe the effects of 100 mM NaCl on their activities in both rice cultivars. The activity of Δ-pyrroline-5-carboxylate reductase in salt-resistant cultivar was increased twice after 5 d in 100 mM NaCl. Simultaneously, the activity of L-proline dehydrogenase was decreased significantly. High activities of Δ-pyrroline-5-carboxylate reductase may be regarded as a biological marker for screening the sensitive and resistant cultivars of rice seed under NaCl-salinity.


Rice Cultivar Proline Accumulation Free Proline Ballygunge Circular Road Resistant Rice Cultivar 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Arnon, D.I., Hoagland, D.R.: The water culture method for growing plants without soil. - Univ. Calif. Agr. Exp. Circ.347: 1–39, 1938.Google Scholar
  2. Aspinall, D., Paleg, L.G.: Proline accumulation — physiological aspects. - In: Paleg, L.G., Aspinal, D. (ed.): The Physiology and Biohemistry of Drought Resistance in Plants. Pp. 206–211. Academic Press, Sydney 1981.Google Scholar
  3. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline in water-stress studies. -Plant Soil39: 205–210, 1973.CrossRefGoogle Scholar
  4. Hoffman, G.J.: Alleviating salinity stress. - In: Arhin.C.F., Taylor,H.M. (ed.): Modifying the Rood Environment to Reduce Crop Stress. Pp. 305–343. American Society of Agricultural Engineers, St. Joseph 1981.Google Scholar
  5. Huber, W.: Über den Einfluss von NaCl- oder Abscisinsä'urebehandlung auf den Protein- Metabolismus und einige weitere Enzyme des Aminosäurestoffwechsels in Keimlingen vonPennisetum typhoides. - Planta121: 225–235, 1974.CrossRefGoogle Scholar
  6. Laliberte, G., Hellebust, A.J.: Pyrroline-5-carboxylate reductase inChlorella autotrophica andChlorella saccharophila in relation to osmoregulation. - Plant Physiol.91: 917–923, 1989.PubMedCrossRefGoogle Scholar
  7. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin-phenol reagent. - J. biol. Chem.193: 265–275, 1951.PubMedGoogle Scholar
  8. Mezl, V.A., Knox, W.E.: Properties and analysis of a stable derivative of pyrroline-5-carboxylic acid for use in metabolic studies. - Anal. Biochem.74: 430–440, 1976.PubMedCrossRefGoogle Scholar
  9. Rena, A., Splittstoesser, W.E.: L-proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotyledons. -Phytochemistry14: 657–661, 1975.CrossRefGoogle Scholar
  10. Sudhakar, C., Reddy, P.S., Veeranjaneyulu, K.: Biochemical changes during early seedling growth of horsegram(Dolichos biflorus L.) subjected to salt-stress. - Indian J. exp. Biol.25: 479–482, 1987.Google Scholar
  11. Treichel, S.: The influence of NaCl on proline accumulating cell suspension cultures ofMesembryanthemum nodiflorum and other halophytes. - Physiol. Plant.67: 173–181, 1986.CrossRefGoogle Scholar
  12. Yoshio, K., Tsuyoshi, O., Yasuhiko, Y., Shigeho, I.: Proline in osmoregulation ofBrevibacterium lactofermentum. - Agr. biol. Chem.53: 2475–2479, 1989.Google Scholar

Copyright information

© Institute of Experimental Botany 1992

Authors and Affiliations

  • D. Roy
    • 1
  • A. Bhunia
    • 1
  • N. Basu
    • 1
  • S. K. Banerjee
    • 1
  1. 1.Department of BiochemistryUniversity College of Science Calcutta UniversityCalcuttaIndia

Personalised recommendations