Folia Microbiologica

, Volume 33, Issue 4, pp 261–266 | Cite as

Elicitor activity ofAgrobacterium radiobacter in plants

  • K. Novák
  • M. Staněk
  • J. Řičica
  • E. Šímová


Agrobacterium radiobacter was tested for the ability to induce the accumulation of phytoalexins and hypersensitive necrotic reaction in pea, bean and potato. A live bacterial suspension with a cell concentration of 1/pL and a solution of a crude polysaccharide produced by the bacteria caused the hypersensitive reaction in potato and bean and the production of phytoalexins in all three species of plants. The results obtained are discussed in connection with the previously found protective effect of the studied strain ofA. radiobacter against soil phytopathogenic fungi. A contribution of defense reactions to the determination of host specificity of the pathogenic strains of theAgrobacterium genus has been proposed.


Sugar Beet Crude Polysaccharide Pisatin Elicitor Activity Potato Tuber Tissue 
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  1. Anand V.K., Heberlein G.T.: Crown gall tumorigenesis in potato tuber tissue.Am. J. Bot.64, 153–158 (1977).CrossRefGoogle Scholar
  2. Azad H.R., Kado C.I.: Relation of tobacco hypersensitivity to pathogenicity ofErwinia rubrifaciens.Phytopathology74, 61–64 (1984).Google Scholar
  3. Bailey J.A., Burden R.S.: Biochemical changes and phytoalexin accumulation inPhaseolus vulgaris following cellular browning caused by tobacco necrosis virus.Physiol. Plant Pathol.3, 171–177 (1973).Google Scholar
  4. Bell A.A.: Biochemical mechanisms of disease resistance.Ann. Rev. Plant Physiol.32, 21–81 (1981).CrossRefGoogle Scholar
  5. Buchholz W.G., Thomashow M.F.: Comparison of T-DNA oncogene complements ofAgrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges.J. Bacterial.160, 319–326 (1984).Google Scholar
  6. Cruickshank I.A.M.: Pisatin studies: the relation of phytoalexins to disease reaction in plants, pp. 325–336 inEcology of Soil-Born Plant Pathogens (K. F. Baker, W. C. Snyder, Eds.). University of California Press, Berkeley 1965.Google Scholar
  7. Cruickshank I.A.M., Perrin D.R.: Studies on phytoalexins. III. The isolation, assay and general properties of a phytoalexin fromPisum sativum L.Austral. J. Biol. Sci.14, 336–348 (1961).Google Scholar
  8. Cruickshank I.A.M., Perrin D.R.: Studies on phytoalexins. XI. The induction, antimicrobial spectrum and chemical assay of phaseollin.Phytopathol. Z.70, 209–229 (1971).CrossRefGoogle Scholar
  9. Dell A., York W.S., McNeil M., Darvill A.G., Albersheim P.: The cyclic structure of β-D-(1→2)-linked D-glucans secreted byRhizobia andAgrobacteria.Carbohydr. Rea.117, 185–200 (1983).CrossRefGoogle Scholar
  10. Dudman W.F., Jones A.J.: The extracellular glucans ofRhizobium japonicum strain 3I1B 71a.Carbohydr. Res.84, 358–364 (1980).CrossRefGoogle Scholar
  11. Garas N.A., Kuć J.: Potato lectin lyses zoospores ofPhytophtora infestans and precipitates elicitors of terpenoid accumulation produced by fungus.Physiol. Plant Pathol.18, 227–237 (1981).Google Scholar
  12. Habgreaves J.A., Bailey J.A.: Phytoalexin production by hypocotyls ofPhaseolus vulgaris in response to constitutive metabolites released by damaged cells.Physiol. Plant Pathol.13, 89–100 (1978).CrossRefGoogle Scholar
  13. Heath M.C., Higgins V.J.:In vitro andin vivo conversion of phaseollin and pisatin by an alfalfa pathogenStemphylium botryosum.Physiol. Plant Pathol.3, 107–120 (1973).Google Scholar
  14. Hooykaas P.J.J., Schilperoort R.A.: The molecular genetics of crown gall tumorigenesis.Adv. Genet.22, 209–283 (1983).CrossRefGoogle Scholar
  15. Keen N.T., KenneDY B.W.: Hydroxyphaseollin and related isoflavanoids in the hypersensitive reaction of soybeans toPseudomonas glycinea.Physiol. Plant Pathol.4, 173–185 (1974).Google Scholar
  16. Klement Z.: Rapid detection of the pathogenicity of phytopathogenic pseudomonads.Nature199, 299–300 (1963).PubMedCrossRefGoogle Scholar
  17. Kuć J.: Plant immunization — mechanisms and practical implications, p. 157–178 inActive Defense Mechanisms in Plants (R. K. S. Wood, Ed.). Plenum Press, New York 1982.Google Scholar
  18. Lyon G.D.: Occurence of rishitin and phytuberin in potato tubers inoculated withErwinia carotovora var.atroseptica.Physiol. Plant Pathol.2, 411–416 (1972).Google Scholar
  19. Metlitskij L.V., Ozeretskovskaya O.L., Saveleva O.N., Dyakov YU.T., Vasyukova N.I., Davydova M.A., Chalova L.I., Chalenko G.I.: Isolation of rishitin and lyubimin in an infection drop placed on the potato tuber surface. (In Russian)Prikl. Bioch. Mikrob.9, 744–752 (1973).Google Scholar
  20. Novák K., Staněk M.: The elicitor activity ofAgrobacterium radiobacter. Abstr. 16th Congr. Czech. Soc. Microbiol., Banská Bystrica 1983;Folia Microbiol.29, 407 (1984).Google Scholar
  21. Pueppkk S.G., Benny U.K.: Adsorption of tumorigenicAgrobacterium tumefaciens cells to susceptible potato tuber tissues.Can. J. Microbiol.30, 1030–1037 (1984).CrossRefGoogle Scholar
  22. Staněk M.: Effect ofAgrobacterium rhizogenes on the growth and health condition of sprouting cucumber and sugar beet plants. Abstr. 13th Congr.Czech.Soc.Microbiol., Špindlerův Mlýn 1976;Folia Microbiol.21, 213 (1976).Google Scholar
  23. Staněk M.: Possibilities of usingAgrobacterium radiobacter B6 for the biological control of sugar beet root rot. (In Czech)Sborník ÚVTIZ — Ochrana rosflin19, 169–181 (1983).Google Scholar
  24. Staněk M., Řičica J., Šímová E., El Shanawani M.Z.: Effect of the polysaccharide ofAgrobacterium radiobacter on the growth of plants and occurence of damping-off in sugar beet.Folia Microbiol.28, 91–99 (1983).CrossRefGoogle Scholar
  25. West C.A.: Fungal elicitors in the phytoalexin response in higher plants.Naturwiss.68, 447–457 (1981).CrossRefGoogle Scholar
  26. Whatley M.H., Margot J.B., Schell J., Lippincott B.B., Lippincott J.A.: Plasmid and chromosomal determination ofAgrobacterium adherence specificity.J. Gen. Microbiol.107, 395–398 (1978).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1988

Authors and Affiliations

  • K. Novák
    • 1
  • M. Staněk
    • 1
  • J. Řičica
    • 1
  • E. Šímová
    • 1
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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