Summary
The present study examined systematically the effect of proglucagon-derived peptides on secretion of the exocrine rat pancreas. It was studied whether glucagon, oxyntomodulin, glucagon (19–29), glucagon (22–29), glucagon-like peptide 1 (7–36)amide, or glucagon-like peptide 2(1, 10, 100, 1000 pM, respectively) alters basal or cholecystokinin-8 (1, 10, 100 pM)-induced amylase release from isolated acini. None of the peptides showed any effect on basal or CCK-stimulated amylase secretion. Furthermore, binding studies utilizing labeled peptides excluded specific binding sites on rat acinar cells for glucagon or glucagon-like peptide 1 (7-36) amide. Our data argue against a direct role for proglucagon-derived peptides in the regulation of exocrine pancreatic secretion in rats.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Williams JA, Goldfine ID. The insulin-acinar relationship. In: The exocrine pancreas, biology, pathobiology, and diseases. Go VWL, Gardner JD, Brooks FP, Lebenthal E, DiMagno EP, Scheele GA (eds). Raven Press, New York, NY 1986; pp. 347–360.
Söling, HD, Unger KO. The role of insulin in the regulation of amylase synthesis in the rat pancreas. Eur. J. Clin. Invest. 1982; 2: 199–212.
Dyck WP, Rudic J, Hoexter B, Janowitz HD. Influence of glucagon on pancreatic exocrine secretion. Gastroenterology 1969; 56: 531–537.
Dyck WP, Texter EC, Labater JM, Hightower NC. Influence of glucagon on pancreatic exocrine secretion in man, Gastroenterology 1970, 58: 532–539.
Shaw HM, Heath TJ. The effect of glucagon on the formation of pancreatic juice and bile in the rat. Can. J. Physiol. Pharmacol. 1973; 51: 1–5.
Iwatsuki K, Ono H, Hashimoto K. Effects of glucagon on pancreatic secretion in the dog. Clin. Exp. Pharmacol. Physiol. 1976; 3: 59–65.
Sommer H, Kasper H. Effect of acetylcholine, gastrin, and glucagon alone and in combination with secretin and cholecystokinin on the secretion of the isolated perfused rat pancreas. Res. Exp. Med. 1981; 179: 239–247.
Singh M. Effect of glucagon on digestive enzyme synthesis, transport, and secretion in mouse pancreatic acinar cells. J. Physiol.(London) 1980; 306: 307–322.
Manabe T, Steer ML. Effects of glucagon on pancreatic content and secretion of amylase in mice. Proc. Soc. Exp. Biol. Med. 1979; 161: 538–542.
Pandol SF, Sutliff VE, Jones SW, Charlton CG, O’Donohue TL, Gardner JD, Jensen RT. Action of natural glucagon on pancreatic acini: due to contamination by previously undescribed secretagogues. Am. J. Physiol. 1893; 245: G703–710.
Conlon JM. Proglucagon-derived peptides: nomenclature, biosynthetic relationships, and physiological roles. Diabetologia 1988; 31: 563–566.
Manaka H, Taniguchi H, Wada K, Takahashi H, Takahashi K, Katagiri T, Sasaki H. Glucagon-like peptide-1 in the rat pancreas. Biomedical Res. 1987; 8: 1–8.
Williams JA, Korc M, Dormer RC. Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am. J. Physiol. 1978; 235: E517–524.
Göke B, Leferink J, Göke R, Adler G. Effect of a low-molecular weight serine proteinase inhibitor (camostate) on amylase release from isolated pancreatic acini. Res. Exp. Med. 1989; 33–38.
Williams JA, Hootman SR. Stimulus-secretion coupling in pancreatic acinar cells. In: The exocrine pancreas: biology, pathobiology, and diseases. Go VWL, Gardner JD, Brooks FP, Lebenthal E, DiMagno EP, Scheele GA (eds). Raven Press, New York, NY, 1986; pp. 21–32.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Weber, V., Fehmann, H.C., Göke, R. et al. Effect of proglucagon-derived peptides on amylase release from rat pancreatic acini. Int J Pancreatol 7, 325–330 (1990). https://doi.org/10.1007/BF02924455
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02924455