Effect of proglucagon-derived peptides on amylase release from rat pancreatic acini
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The present study examined systematically the effect of proglucagon-derived peptides on secretion of the exocrine rat pancreas. It was studied whether glucagon, oxyntomodulin, glucagon (19–29), glucagon (22–29), glucagon-like peptide 1 (7–36)amide, or glucagon-like peptide 2(1, 10, 100, 1000 pM, respectively) alters basal or cholecystokinin-8 (1, 10, 100 pM)-induced amylase release from isolated acini. None of the peptides showed any effect on basal or CCK-stimulated amylase secretion. Furthermore, binding studies utilizing labeled peptides excluded specific binding sites on rat acinar cells for glucagon or glucagon-like peptide 1 (7-36) amide. Our data argue against a direct role for proglucagon-derived peptides in the regulation of exocrine pancreatic secretion in rats.
Key WordsProglucagon oxyntomodulin glucagon glucagon-like peptide 1 & 2 glucagon 22–29 isolated pancreatic acini rat amylase secretion
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