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International Journal of Pancreatology

, Volume 4, Issue 1, pp 13–27 | Cite as

Measurement of pancreatic enzyme synthesis in humans

Problems in patients with calcific pancreatitis
  • Stephen J. D. O’Keefe
  • Janice M. Ogden
  • Gerardine O. Young
  • Joy Dicker
  • Andrew H. Gird wood
  • I. N. (Solly) Marks
Article
  • 21 Downloads

Summary

Earlier studies have suggested that the rate of incorporation of labeled amino acids into duodenal juice proteins during pancreatic stimulation may be used to calculate pancreatic enzyme synthesis and function. In the present study, a pulse/4 h continuous intravenous infusion of14 C labeled leucine was used to compare synthesis rates in 6 patients with chronic calcific pancreatis(CP) to 4 controls. Analysis of duodenal juice protein demonstrated a delay of approximately 1 h in the appearance of labeled proteins, followed by a linear increase in specific activity, allowing calculation of synthesis that varied between 2.6–2.8 h in controls and 6–48 h in CP. The protein in controls was representative of enzyme protein, but that of CP was not, since it was heavily contaminated with albumin (up to 50%). The results indicate that enzyme secreted during the first hour of stimulation is derived from pancreatic stores and that the synthesis rate of enzymes secreted thereafter is approximately 2.7 h in normal humans. The method was, however, unable to determine rates in patients with CP owing to heavy contamination of enzymes with exudative proteins.

Key Words

Pancreatic enzyme synthesis pancreatitis 

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References

  1. 1.
    Boyd EJS, Wood H, Clarke G, Neill GD, Hutchinson F, Wormsley KG. Pancreatic synthesis rates: A new test of pancreatic function. Scand. J. Gastroenterol. 1982; 17: 225- 231.PubMedCrossRefGoogle Scholar
  2. 2.
    O’Keefe SJD, Ogden J, Dicker J, Boyd E, Marks IN. Evaluation of the method of constant I V infusion of labelled amino acids in the measurements of pancreatic enzyme synthesis rates. Dig. Dis. Sci. 1986; (10): 360S, (1428).Google Scholar
  3. 3.
    Waterlow JC, Garlick PJ, Millward DJ. Total protein turnover from measurements on plasma and on the whole body. In Protein turnover in mammalian tissues and in the whole body. Amsterdam Elsevier North-Holland, chapter 8: 1978, 301–326.Google Scholar
  4. 4.
    O’Keefe SJD, Moldawer LL, Young, VR, Bilmazes C, Blackburn GL. The influence of intravenous nutrition on protein dynamics following surgery. Metabolism 1981; 30: 12: 1150–1158.PubMedCrossRefGoogle Scholar
  5. 5.
    Waterlow JC. Lysine turnover in man measured by intravenous infusion of L(U14C) lysine. Clin. Sci. 1967; 33: 507–515.PubMedGoogle Scholar
  6. 6.
    Matthews DE, Motil KJ, Rohrbaugh DK, Burke JF, Young VR, Bier DM. Measurement of leucine metabolism in man from a primed, continuous infusion of L(1-3C) leucine. Am. J. Physiol. 1980; 238: (5): E473–479.PubMedGoogle Scholar
  7. 7.
    Simon O, Zebrowska T, Bergner H, Miinchmeyer R. Investigations on the pancreatic and stomach secretion in pigs by means of continuous infusion ofl4C-amino acids. Arch. Tierenahr. 1983; 33: 1: 9–22.Google Scholar
  8. 8.
    Schwert GW and Takenaka Y. A spectrphotometric determination of trypsin and chymotrypsin. Biochimica Biophysica Acta 1955; 16: 570–575.CrossRefGoogle Scholar
  9. 9.
    Pimstone NR. A study of the starch-iodine complex: a modified colorimetric micro determination of amylase in biologic fluids. Clin. Chem. 1964; 10: 891–906.PubMedGoogle Scholar
  10. 10.
    Weber H. Mikromethode zur bestimmung der pankreaslipase im serum. Deutsch Med. Wsch. 1965; 90: 1170–1174.CrossRefGoogle Scholar
  11. 11.
    Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein, utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 72: 248- 254.PubMedCrossRefGoogle Scholar
  12. 12.
    Robberecht P, Cremer M, Christophe J. Discharge of newly synthesized proteins in pure juice collected from the human pancreas. Indication of more than one pool of intracellular digestive enzymes. Gastroenterol. 1977; 72: (3): 417–420.Google Scholar
  13. 13.
    Rothman SS, Isenman LD. Secretion of digestive enzyme derived from two parallel intracellular pools. Am. J. Physiol. 1974 226: 1082–1087.PubMedGoogle Scholar
  14. 14.
    O’Keefe SJ, Abraham R, El-Zayadi A, Marshall W, Davis M, Williams R. Increased plasma tyrosine concentrations in patients with cirrhosis and fulminant hepatic failure associated with increased plasma tyrosine flux and reduced hepatic oxidation capacity. Gastroenterol. 1981; 81: (6) 1017–1024.Google Scholar
  15. 15.
    Multigner L, Figarella C, Sarles H. Diagnosis of chronic pancreatitis by measurement of lactoferrin in duodenal juice. Gut 1981; 22: (5): 350–354.PubMedCrossRefGoogle Scholar
  16. 16.
    Clemente F, Ribeiro T, Colomb E, Sarles H. Comparison des proteines de sucs panreatiques humains normaux et pathologiques. Dosage des proteines seriques et mise en evidence d’une proteine particuliere dans la pancreatite chronique calcifiante. Biochem. Biophys. Acta 1971; 251: 456–466.PubMedGoogle Scholar
  17. 17.
    Scheele G, Bartelt D, Bieger W. Characterization of human exocrine pancreatic proteins by two-dimensional isoelectric focusing/sodium dodecyl sulfate gel electrophoresis. Gastroenterol. 1981 80: (3): 461–473.Google Scholar
  18. 18.
    Tasso F, Stemmelin N, Sarles H, et al. Comparative morphometric study of the human pancreas in its normal state and in primary chronic calcifying pancreatitis. Biomedecine 1973; 18: 134–144.PubMedGoogle Scholar

Copyright information

© Humana Press Inc 1989

Authors and Affiliations

  • Stephen J. D. O’Keefe
    • 1
    • 2
  • Janice M. Ogden
    • 1
    • 2
  • Gerardine O. Young
    • 1
    • 2
  • Joy Dicker
    • 1
    • 2
  • Andrew H. Gird wood
    • 1
    • 2
  • I. N. (Solly) Marks
    • 1
    • 2
  1. 1.Gastro-intestinal ClinicGroote Schuur Hospital Observatory
  2. 2.University of Cape TownSouth Africa

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